The protective effect of alcoholic extract of Zingiber officinale Rosc. (Ginger) on body weight , fertility and some of biochemical parameters of blood at females albino rats that treated with Paracetamol (Acetaminophen) drug.

Saher Mahmood Jwad

Magazine of Al-Kufa University for Biology, 2015, Volume 7, Issue 2, Pages 0-0

The present research was performed in the animal house of biology department / Faculty of Education for Girls, aimed to study the effect of alcoholic extract of Zingiber officinale Rosc. on body weight , fertility and some of biochemical parameters of the blood at females albino rats that treated with Paracetamol drug, and included the follow up (32) females of albino rats were sexually matured at three months age. For this study to carry out, the animals were divided randomly into four groups, eight females for each group, the first group (I) was given normal saline as a control group, the second group (II) was given the concentration( 7.5) mg/kg of Paracetamol drug, while the third group (III) was first given the concentration(500) mg/kg of alcoholic extract of Zingiber officinale Rosc. , and after one hour the same group was given Paracetamol drug at the concentration(7.5)mg/kg. As while as the forth group (IIII) was given Zingiber officinale Rosc. alcoholic extract at the concentration( 500 )mg/kg. The dosage process was continued orally for 21 days (for five respective estrus cycles) once daily. The results were revealed: the dosage with Paracetamol drug was showed a significant decrement (P < 0.05) in body weight, ovary weight, ovarian follicles diameters and their numbers, concentrations of gonadotropin releasing hormones (FSH and LH), ovarian hormones (estrogen and progesterone), thyroid hormones (T4 and T3), erythropoietin and glutathione (GSH) in the serum. In the contrast of that the treatment with drug was caused a significant increment (P < 0.05) in concentrations of thyroid stimulating hormone (TSH) and malondialdehyde (MDA) in comparative with the control group and other groups.
On the other hand, the injection with alcoholic extract of Zingiber officinale Rosc. was showed antagonistic effect to the physiological action of Paracetamol drug through the significant increase (P < 0.05) in: body weight, ovary weight, ovarian follicles diameters and their numbers, concentrations of: FSH, LH, estrogen, progesterone, T4, T3, erythropoietin and GSH in the serum, and the significant decrease (P < 0.05) in concentrations of TSH and MDA when compared with the control group and the group that treated with Paracetamol drug.
The present study was concluded to: the alcoholic extract of Zingiber officinale Rosc. was revealed a protective effect on cells and body tissues especially ovaries of rat females which treated with Paracetamol drug, so we recommend to use it as a nutritional or remedial supplement at the treatment with Paracetamol drug.

* Study of bacterial species associated with cases of infertility in men in the province of Najaf.

Kais Khudhair Abdulabbas ALhadrawi; Siham Jasim Mohsen AL-Kaabi; Ihsan Edan .AL-Saimary

Magazine of Al-Kufa University for Biology, 2015, Volume 7, Issue 2, Pages 29-42

A total of 328 seminal fluid samples were collected from infertile patients with various types of infertility; Azoospermia, oligozoospermia, asthenozoospermia, teratozoospermia aged between 19 to 60 years, in comparsion with control group (Normospermia) during march until August 2013. The biggest number of infertility were in age 19-29 years which reached 143(44%) ,followed by 30-39 years 109 (33%) and ˃ 40 years 76(23%) . There are significant differences between various infertility types and age groups (p˂0.05).
A total of 48 isolations of Gram positive and negative bacterial Types were isolated Staphylococcus aureus has a highly prevalence from (45) infertile patients numbered 15 isolates in percentage (29%) , followed by other bacterial types : Streptococcus pyogenes , Klebsiella Spp. , Escherichia coli, Enterobacter Spp. and Neisseria gonorrhea that numbers of isolations and percent ages as follows: 14(29%),7(15%) ,3 (6%),and 2(4%) respectively . statistically, there are significant differences between numbers of bacterial isolates (P˂0.05).

Evaluate the effectiveness of ethanolic extracts of Iraqi propolis and honey bee pollen in the viability of the protoscolices of Echinococcus granulosus In Vitro Iraqi vital for primates initial parasite Echinococcus granular in Echinococcus granulosus Glass In vitro

jameel geriy yosef

Magazine of Al-Kufa University for Biology, 2015, Volume 7, Issue 2, Pages 43-51

The present study aimed to examine the effectiveness of extracts of Alaithanolah Bropouls Iraqi and pollen in honey bees Iraqi vital for primates initial parasite Echinococcus granular in Echinococcus granulosus Glass In vitro. The results showed that the extracts Alaithanolah for Bropouls Iraqi and pollen bees Iraqi honey is very effective in the destruction of the initial primates of the parasite Echinococcus granular, and that the loss ratio increases with concentration and duration of exposure to both extracts, and when the transaction focus (6) mg / ml of each of the extracts was the proportion of decimation primates 100% after 15 minutes of exposure to extract pollen honey bees and after 20 minutes when exposed to extract Albropouls.kma results showed that the extract of pollen bee honey was more effective in the destruction of primates from Albropouls extract, where it was necessary focus for the destruction of 50 % of the initial primates (0.39) mg / ml and (0.57) mg / ml, respectively. I conclude from this study that the pollen and honey bees Albropouls natural as materials that can be used to kill primates for the initial parasite Echinococcus granular.

Effect of Nigella sativa extract in the activity of thyroid gland in male rats Ihsan Raisan Ibrahim

Ihsan Raisan Ibrahim

Magazine of Al-Kufa University for Biology, 2015, Volume 7, Issue 2, Pages 317-322

This study carried out to determine the effects of Nigella sativa extract in the function and structure of thyroid gland in male rats . Twenty male rats were distributed randomly to four groups .First group administrated orally with distelled water and considerd as a control group , while the other groups administrated extract of Nigella sativa at dose ( 25, 50 , 100 ) mg / kg body weight repectively Animals were anesthized and the samples of blood were collected for determine leveles of T3 , T4 and TSH . Thyroid gland were removed for preparation the histological sections .

Results of this study demonstrated that there were significant increase in weight of thyroid gland in second and third groups compured with a control groups . Also there was high increase in T3 level second and third groups . compared with control and group , while there was non significant difference in T4 level in all groups compared with a control group . Results showed there was significant decrease in TSH level .

Histolosical study showed significant decrease in the height of epithelial cells in second and third group compared with acontrol group, while there was significant increase in the diameter of follicle and the colloid in the second and third group compared with control group .

Magnetic treatment of water for germination in the germination of the seeds of broccoli effect Brassica oleraceavar. botrytis Class Solid snow

kouthar A. Al-jabbar; thamer K. M

Magazine of Al-Kufa University for Biology, 2015, Volume 7, Issue 2, Pages 120-131

A I ran this experiment at the Department of Life Sciences / College of Science / University of Kufa in Najaf province and included the planting of seeds for the purpose of measuring the germination percentage for the duration of 1/8 up to 08/08/2011 Included experiment study the effect of two factors: the first factor of five intensities for processing magnetic irrigation water (0500750, 1500.2500 gauss) by magnetic treatment devices Magnetrons bipolar dipolar, second factor for germination, and Tdakhlathma in the germination of the seeds of cauliflower class Solid snow. Duncan test polynomial was used (DMRT) compared to averages in the global experience and the correlation coefficient to indicate the relationship between the germination percentage over time. The most important results can be summarized as follows:
• chemical analysis of water for irrigation showed that magnetic treatment process affected the physical properties of water and did not much affect the existing concentration of the ions.
• it was to use magnetic treatment of water for irrigation significant effect in raising the percentage of germination of the seeds of class Cauliflower (Solid snow) in comparison with the comparison with the highest percentage of germination of seeds after irrigation water processor magnetically strongly in 1500 gauss in the two trials of laboratory and field has reached 71.14 and 65.62%. respectively, while the ratio stood at 56.67 and 52.19%, respectively, in the treatment of comparison.

A study of the capability of Aspergillus species(A.niger, A.flavus and A.terreus ) to produce phospholipase enzyme phenotypically

wefaq Ahmed; sameira kadhum

Magazine of Al-Kufa University for Biology, 2015, Volume 7, Issue 2, Pages 219-224

This study included 113 ear swabs from patients (males and female ) with Otomycosis , visited the otolaryngology consultation room at Al-Sader Medical City in Al-Najaf / Iraq in addition to the private otolaryngology clinics of some of ear,nose and throat specialists , during the period from October 2012 to March 2013 . aims to isolate and diagnose the causative opportunistic mycotic infection of the ear and to identify the enzymatic activity of phospholipase phenotypically as an important virulence factor of fungi .
One hundred and three positive swabs were obtained including "Cephalosporium ,Candida spp, Aspergillus spp, Neurospora (Monilia ) and Trichosporium beigelii .
A.flavus and A.niger showed phospholipase activity in two degrees (++ ,+++) , but A.terreus shoed only (++) .

Investigation of some virulence factors of P. aeruginosa bacteria isolated from different sources in a hospital Afak in Iraq.

Ali Abd Raheam Alnashi; Zainab Abd Alkaream

Magazine of Al-Kufa University for Biology, 2015, Volume 7, Issue 2, Pages 306-315

Samples were collected study of hospital Afak General , taken from sources of environmental and clinical for the period from the first of November 2012 until the end of April 2013 and by 400 clinical specimens and environmental, the number of samples that gave growth Positive (236) sample and (164) sample gave a negative growth .
The number of isolates total bacteria P. aeruginosa in this study (30) isolation rate of (12.7%) of the total number of samples, the number of isolates environmental (25) isolates were distributed among (3) isolation was sourced lobbies premature infants, (11) isolation source lobbies entry of patients, (4) isolation sourced cuisine, (6) isolation sourced Eat surgical operations, (1) isolation sourced hands and clothes, workers and the number of isolates of clinical (5) were distributed among (2) isolated from wounds and (3) the isolation of burns .
Tested the sensitivity of all isolates of P. aeruginosa direction of 13 antibiotic by reference to the way the spread of disks; as it gave the highest resistance (90%) of the anti Tobramycin, (80%) of the anti Cefotaxime, (73%) of the anti Titracycline, (63%) of the anti Naldixic acid, (60%) of the anti Trimethoprim, (50%) of amadada piperacilin and Aztronam, (40%) of the anti Ticarcillin and Nitrofurntine and (33%) of the anti Impenem and Ciprofloxacin resistance was the lowest rate (23%) of the anti Gentamycin .
Was to investigate the ability of some bacteria P.aeruginosa Virulence factor where he found that all isolates have the ability full (100%) on the production of enzymes haemolysin , protease and gelatinase and have the ability to produce Slime layer by (33.33%) .

Protein pattern changes and determination salt tolerant proteins of okra (Abelmoschus esculentus Moench.) plants irrigated with salty water and treated with potassium nitrate and Tocopherol

Essam H. A. Al- Doghachi; Awatif N. Jerry; Faris I. Obead

Magazine of Al-Kufa University for Biology, 2015, Volume 7, Issue 2, Pages 265-276

An experiment was conducted in a private orchard at Shat-Al- Arab District, Basrah Governorate during the summer seasons of 2011and 2012 to study the effect of irrigation water quality and spraying with potassium nitrate and Tocopherol on protein pattern changes of okra plants.
Treatments included three sources of water i.e. tab water, tab water + river water and river water alone or with 30 m. mol. potassium nitrate and150mg. L.-1Tocopherol. Result showed that the total number of protein bands/lane did not change under the low salt concentration but was dramatically reduced by the high salt treatment. The sum of optical densities (O. D.) of protein bands was inhibited by both levels of irrigation water, but was induced with the addition of KNO3 and Tocopherol with tab water+ river and with river water. different polypeptides of molecular weights were completely disappeared under river water irrigation. These bands reappeared in response to the addition of KNO3 and Tocopherol. Moreover, the optical density of every individual protein band was induced by Tocopherol under the low salinity. Results indicate synergistic interaction between salinity stress and KNO3 and Tocopherol for the sake of salt tolerance in okra plants.