Changes in Viability,Vigor andRelative Growth Rate (RGR), in Okra(AbelmoschusesculentusL.)seeds during accelerated Ageing Technique

Muhannad.M.Sahib

Magazine of Al-Kufa University for Biology, 2014, Volume 6, Issue 1, Pages 96-104

Reduction of seedling growth is a consequence of seed deterioration. An experiment was conducted to evaluate the effects of duration of seed aging on Okra (AbelmoschusesculentusL.) seeds germination characteristics.Experiment conducted as completely randomized design with 3 replications. Seeds were subjected to accelerated aging treatment for, 3, 7 and 10 day at 45 ±1 C° and 100% relative humidity. These artificially aged seeds were compared to control (Unaged seeds) for evaluation of seed vigor.Accelerated aging of Okra seeds up to three days had significant effect on germination percentage. Increase ageing period caused highly seed moisture content, electrolyte leakage (in term electrical conductivity EC) and decreased in germination percentage. Germinability waslost completely at 10 days of ageing. The accelerated ageing caused in addition of reducing germination percentage, they caused reduction in seedling length, seed vigor index, germination speed index,and shoot, rootfresh & dry weight.Finally, the results revealed that accelerated aging caused depression of Okra seeds viability through the above parameters

Cinnamic acid activity towards Trichlorfon pesticide and liver function enzymes in rat.

Mousa; Nibal; Saad; Khudiar; A.Muhsin; Eman; Shahad; Sabbar; Ab. Ahmed; Ishrak

Magazine of Al-Kufa University for Biology, 2014, Volume 6, Issue 1, Pages 1-9

The study was carried out to determine hepatotoxicity and hepatoprotcetive effects for cinnamic acid in comparison with vitamin C against the mutagenic pesticide influence of trichlorfon ,which is a chemical compound that damage hepatic cells and has mutagenic effects .The effect was studied in mammalian system invitro depended on evaluating the enzymatic activity of three hepatic enzymes: Alanine Transaminase(ALT),Aspartate Transaminase (AST),Alkaline Phosphate (ALP).Two concentration of pure cinnamic acid (60 ,30) mg/kg were evaluated to chose the suitable concentration which remembered the negative control. In order to used in the interaction experiments, included two types to treatments pre-trichlorfon and post-trichlorfon in order to determine the mechanisms of the pure cinnamic acid showed no toxic and hepatotoxicity influence in biological system and instead it showed highly performance in preventing or reducing the hepatotoxicity of trichlorfon.Cinnamic acid increased the ALT,AST and ALP enzymes with nutral levels especially in dose 30 mg/kg .The positive effect was higher were pure cinnamic acid was used as post-trichlorfon treatments and to less extent in pre-trichlorfon treatments, therefore , cinnamic acid can be considered as a cure hepatocytes from acute liver damage at first degree and responsible at the second degree as a cardiac ,skeletal muscle and placental tissue protective.

Comparability Study between Digital and Visual Colorimeter by using of Chlorine Indicators of DpD No.1 and DpD No.3 under practical conditions

Fulath Abdul-RedahMuhsin

Magazine of Al-Kufa University for Biology, 2014, Volume 6, Issue 1, Pages 10-22

All public drinking water system was required to measure the levels of residual chlorine in a practically accurately instruments to ensure that the water was adequately disinfected. This experimental study was carried out to compare under practical condition in laboratory the test results of free and total chlorine in the water samples between digital colorimeter and visual colorimeter by using of chemical indicatorreagentsof ((DpD number (1)) for testing of free chlorine in water samples, and ((DpD number (3)) for testing of total chlorine in water samples. The experiments lasted from (1/10/2011 to 1/5/2012). The study was conducted in the Laboratories of Al-Kufa Technical Institute in Al-Kufa city that belong to Al-Najaf governorate about 165 Km to the south of Baghdad, and 60 km to the south of Babylon governorate.
200 experiments were done in the laboratory, by made of 2 experiments in each day through a standards laboratory conditions.
There was a highly statistical significant difference (p=0.000), in comparing between digital calorimeter and visual colorimeter of both free and a total chlorine test results.
There was a highly differences in the mean when was comparison of the digital colorimeter with the visual colorimeter of both all free and total chlorine experiment.
There was a strong direct correlation between digital colorimeter experimental results and contact time in both all free and total chlorine experiments, while there was a weak direct correlation between visual colorimeter and contact time in all free and total chlorine experiments.
From the all tested results of experiment that was derived from this study, the study recommend to practically adaptation of a digital colorimeter instead of visual colorimeter in all the chlorine residual test results that was done by ministry of health inspectors in all governorates in monitoring of drinking water, and for more accurate and sensitive test result we recommend to adapted of digital colorimeter in all researches when tracing of chlorine residual of different water samples.

Role of Glutathione in male infected with giardiasis

Maysoon K. A .Al-Hadraawy

Magazine of Al-Kufa University for Biology, 2014, Volume 6, Issue 1, Pages 111-115

The study was conducted 69 out patients and 30 healthy males to determine the influences of infected with Giardia lamblia on levels of Glutathione in males infected with G. lamblia in compared with healthy group. Who have visited Al-Sadder medical city and Al-Hakeem Hospital in Al- Najaf Province during the period from January till August 2012.Diagnosis infection with this parasite by using the wet amount microscope for stool from patients. The results showed significant decrease (P<0.05) in Glutathione in G. lamblia infection patients in compared to control group.

Study of hematological changes and hypertension in patients with hyperthyroidism

Adday; Lika

Magazine of Al-Kufa University for Biology, 2014, Volume 6, Issue 1, Pages 156-161

Hematological abnormalities, have been described in hyperthyroidism. There is interaction among hypothalamus gland , pituitary gland and thyroid gland system in regulation of metabolic and function of body , this achieved by balance between hormonal stimulation with drawl of other hormone by feed back mechanism , any changes in this hormonal balance lead to thyroid gland disorders , the present study was carried out to evaluate the effect of hyperthyroidism on some physiological blood properties is Hb ,WBC ,RBC ,blood pressure ,determination the concentration of thyroid hormones (T3, T4, TSH) for patients ( N=30) which compared with control group (N=30) . The following finding were obtained :
1- Significant decrease (P< 0.05 ) in RBC patients compared with control group.
2- Significant elevation (P<0.05 ) of (T3 , T4 ) concentration and reduction of (TSH) concentration for patients group compared with control group .
3- Significant decrease (P< 0.05 ) in Hb concentration for study group compared with control group .
4- Significant decrease (P< 0.05 ) in WBC patients compared with control group.
5- Significant elevation (P< 0.05 ) in blood pressure patients compared with control group.

Effect of Bitumen Emulsion Application in Water Movement by Capillarity in Different Textures of soil

Sabah Shaffi Al-Hadi

Magazine of Al-Kufa University for Biology, 2014, Volume 6, Issue 1, Pages 22-39

In order to investigate the possibility of reducing the main harmful effects of saline water movement by capillarity in degradation of soils structure and salts accumulation on their surface, an experiment was conducted to evaluate effect of the Bitumen emulsion application on the mechanism movement of different qualities of water by capillary action in three different soils texture (clay, sandy loam and sandy soils), air dried and passed through (2) mm sieve. Half of each soil been mixed thoroughly with Bitumen emulsion of (1%) on dry soil bases and the other half was left without treatment. Soils have been placed into fifty four glass tubes of (100) cm length and (0.8) cm diameter. Three qualities of water (tap water of 1.85 dS m-1, mixed water of 6.22 dS m-1 and drainage water of 10.48 dS m-1) were used. All treatments have been done three times. The height of rising water by the capillarity (cm) at time of measurement (minute) was measured and capillary water velocity (cm h-1) was calculated for all treatments (Bitumen, soils and water qualities). All obtained data were statically analyzed by using the SPSS program.
From using the tab, mix and drainage qualities of water, results of the capillary action indicate differences in the water rising values with the different soils and Bitumen treatments. Increasing of salts concentration in the water caused significant decreases in the capillary water rise values throughout the time of measurements of all treatments. The application of Bitumen caused large reduction in cumulative capillary rise and capillary water velocity values at the start and the end of the capillary action time, the reduction percentage values was 70.08% at the beginning and 12.66% at the end of the measurement time. The reduction in upward movement of saline water by capillary action was recorded in all experiment treatments. The results concluded that, coating of Bitumen emulsion to the soil particles and its penetration into aggregates of the soils caused an improvement in the soil structure units and porosity. These newly formed units of soil structures were not affected by the salt level present in the rising water by capillarity. The application of Bitumen caused increasing in the macro pore spaces (drainage pores) and reduction in the micro pore spaces (capillary pores) of the soils, which, consequently, resulted in a decline in rising movement velocity of saline water by capillarity in all soils.

Effect of Body Mass Index on sperm and semen parameters for infertile Patients

Samah A.AL-Ubaidy; Muhsen K.AL-Murshedy; Saheb Y. AL-Murshedy

Magazine of Al-Kufa University for Biology, 2014, Volume 6, Issue 1, Pages 40-52

Out of 130 specimens of seminal fluid were collected from patients suffering of infertility , after abstinence of 3-5 a period of days from Fertility Center Laboratories Al – Saader Medical City at Al-Najaf Province ,the study continuouse from 172011 to 192011. The aims of this study at clarifying the role of the body weight on infertility patients suffering from Normospermia , Asthenospermia ,Oligospermia ,Azoospermia ,and Teratospermia ,by calculated the Body Mass Index (BMI) for each patients as kg m2 which classify to normal weight and over weight .
The results of this study revealed an increased of percentage Oligospermia percent with BMI of patients in comparison to that of other infertility types , as well as ,it showed clear effect of increase BMI to Normospermia and Asthenospermia patients which it significant decrease (P<0.05) of sperm motility, morphology and concentration of sperm compared between normal and over weight. The study results had revealed significant decrease (P<0.05) of sperm concentration , liquificative time of semen, motility and normal sperm morphology of Oligospermia suffering for increase BMI (over weight). In Azoospermia and Teratospermia patients, the results of sperm and semen parameters revealed a non significant (P>0.05) correlation ships between normal and over weight.
It can be concluded that BMI correlated with reproductive physiology through increase of infertility men percent , when infertile men with high BMI typically are found to have an abnormal semen analysis represented by decrease in sperm concentration, decrease in sperm motility percent , as well as, increase of abnormal forms of spermatozoa.

Epidemiologic study of Dermatophytosis in Al-Najaf government

Dr. Adnan Hamad Al-Hmadani; Dr.Muhsin Abdulhusien Al-Dhalimi; Muhammad Muhsien Abdulhusien Alrufae

Magazine of Al-Kufa University for Biology, 2014, Volume 6, Issue 1, Pages 53-67

Dermatophytosis, commonly referred to as ring worm which, is a superficial fungal infection caused by filamentous fungal species (Dermatophytes) belonging to three anamorphic genera, Trichophyton sp., Microsporum sp. and Epidermophyton sp. It is the most common superficial fungal infection in the world. Due to the importance of high prevalence rate of above diseases, this study was conducted to determine the epidemiologic condition of diseases in Al-Njaf province. Total of 216 specimens of (skin scrappings, hair fragments and nail clippings) were collected. Also 138/216 (63.9%) specimens exhibited positive results in both direct examination and culture, and 32/216 (14.8%) specimens were negative in direct examination and positive in culture therefore only 170/216 (78.7 %) specimens yielded growth of dermatophytes isolates and considered as positive specimens. Microsporum canis showed highest frequency in 54/216 (31.8 %) isolates, while M. audouinii and Trichphyton schoenleinii in 8/216 (4.71%) isolates showed lowest frequency. Also 38/216 (22.4%) of M. canis isolates were in male while 16/216 (9.41%) isolates in female, both T. schoenleinii and M. audouinii were not recorded in female 0(0%). Tinea corporis was the predominant infection in 75/157 (47.77%) patients followed by Tinea capitis in 29/157(18.47%) patients. Gender is not statically significant with dermatophytosis, while a significant (P<0.01) correlation was observed with age, residency variables. Age revealed significant effect (P<0.05) on the distribution of dermatophytosis patients, the age bracket 1-10 years were highest frequency with 42/157(26.8%) patients, while age bracket 61-70 years were lowest frequency 2/157(1.3%) patients. Residency revealed non significant association in relevance with type of dermatophytosis, while significant (P<0.01) correlation was observed with gender. This study showed that there is a high prevalence rate of Dermatophytosis in Al-Najaf province. Therefore, due to importance of the disease, it is necessary to diagnose and cure the disease immediately.

Diagnosis of Brucella melitensis infection in goats milk by milk ring test& Polymerase chain reaction

Ali Anok Najum

Magazine of Al-Kufa University for Biology, 2014, Volume 6, Issue 1, Pages 198-201

B. melitensis is a more infectious zoonotic agents infect human and animals, 120 suspected brucellosis milk samples from goats with history of abortion were collected from Al-Samawa city tested with Milk ring test and Polymerase chain reation technique, results showed 11(9.16%) samples were positive for MRT, while 5(4.16%) samples were positive for PCR technique.

Almaekerobah study the sensitivity of local isolates of bacteria Ps.aeruginosa against anti Norfloxacin

firas nabeeh jaffer

Magazine of Al-Kufa University for Biology, 2014, Volume 6, Issue 1, Pages 202-211

Was obtained on 51 isolated bacteria belonging to Ps.aeruginosa of different clinical samples, including blood, urine and sputum inflammatory wounds and burns and infections from various hospitals in the city of Baghdad for the period from 01/02/2012 up to 03/01/2012 to investigate bacteria showed Ps.aeruginosa test examination results Allergies against six types of antibiotic resistance all isolates to anti Ticarcilin / Clavulanic acid were 96.07% of the isolates were resistant to anti Oxacillin% and 76.4 resistant to anti Azlocillin The percentage of resistance to anti Norfloxacin% 39.21, as well as resistance to anti Levofloxacin% 27.45 percentage emerged while the lowest proportion of resistance to anti Piperacillin / Tazobactam (% 25.49), focus damper minimum values ​​ranged (MICs) of the Anti Norfloxacin isolates that have shown resistance to this counter (1024-128) mcg / mL, were detected over the susceptibility of isolates to produce Baktriossin manner tablets Alacar Cup disc)), were obtained Azltan on producing Bactriossin of the total (10) and the isolation of 20%, and was Aezzltan producing Bactriossin sensitive to anti Norfloxacin.