Phytophthora infestans and evaluate their efficiency in the protection of seeds and tomat plants

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 1-6

The study was carried out to determine the effect of aquatic extracts of some plants including Myretus communis , Ocimum bacilium and Mentha spicata on the growth of pathogenic fungus Phytophthora infestans and to protect tomato plants from infection by this fungus. The results showed the ability of extracts in the inhibition of fungal growth at different levels of each extract and each concentration in same extract. Where the aquatic extract of Myretus at 5% caused the highest rate of inhibition of fungal growth and increased the germination percentage to 73%, and reduced the percentage of seedling damping-off to 4.4% as well as increase in fresh weight and dry of shoot and root, followed by the Mentha extract and Ocimum extract at 50% and 75% respectively as compared to the treatment of control (B).

The susceptibility some testing of rice infection by Fusarium solani and its biological control .

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 7-18

This study was conducted in plant pathology / College of Agriculture / University of Kufa in 28/6/2010 e the effect of using the fungus A.niger to control Fusarium solani. Results showed that the fungus reduce the emergence percentage for rice varieties in this study as the concentration gave 60.33% respectively compared with control treatment that gave 80.33% respectively, also affected in number of rot seeds which was 3.67, respectively compared with control treatment that gave 1.7, respectively. Results showed also that the A.niger have encourage effect on emergence that was 90% respectively compared with control treatment that was 80.11% respectively, and also reduce the number of rot seeds that was 1, respectively compared with control treatment that gave 1.89, respectively. The fungi A.niger reduced the dead seedling and increasing the length of plant compared with control treatment; meanwhile, F. solani increased the dead seedling pre and post-emergence and decreased the plant length.The interaction treatments between the pathogenic fungi and bioagent fungi showed different effects, that the fungi increase the emergence percentage that were 50.67% respectively when compared with the fungi F.solani that gave 60.67%, also the interaction reduced number of rot seeds and increased the plant length.

Comparative study of the heamatological changes in the blood of pregnants and abortus women in Baghdad hospitals

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 26-35

The concentration level of hemoglobin, packed cell volume , total number and differential of white blood cells were studied in 60 pregnant women in different stages of pregnancy.The period of pregnancy were determined by the date of the first day of last menstrual period and 60 aborted at different stages of pregnancy and 40 women non- pregnant and have no previous abortions had used as a control ,were frequent Nu'man Hospital, a maternity top, Tigris NCB, NCB Waziriya in Baghdad province for the period from February until September 2010 Study groups were divided into five age categories and a difference of five years between each category and the next. The most frequent pregnancy was seconded in the age group of (26-30 years ),while the most frequent abortions were see among the age groups of (26-30 years) and (31 - 35 years) by 26.7% and 25% respectively, while for the control group, the third age group (31-35 years old) were the most frequent by 40%, and less number of pregnant women, and abortus and control was in the age group the last (41 years old - above) and 5% of pregnant women and 8.3% of abortus 7.5 of cont Results of this study that pregnant women are more group suffered from a lowest of the values of hemoglobin on the standard value, amounting to% 61.7, as well as a decrease in packed cell volume less than (36%), and age group of pergnants (36-40 years )is the category which saw the lowest 85.7%. The total number of white blood cells in the peripheral blood of pregnant women, and abortus showed a significant increase when compared with women control, which was within the normal range . On the other hand the pregnant showed increasing the number of neutrophils 25% and in the abortus the increase was in the number of monocytes 45% while the proportion was 20% cells of the neutrophil

Study of Some Physiological and Biochemical Changes in Patients with Chronic Renal Failure (CRF) Undergoing Hemodialysis in Al-Najaf Governorate

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 36-44

The current study aimed at assessing some physiological and biochemical changes in patients with chronic renal failure (CRF) undergoing hemodialysis . It was achieved at Al-Hakim general hospital in the Holy city of Najaf during the period from 172010 to 112011 , (100) cases of both sexes were monitored including (75) patients with chronic renal failure and a control group of (25) healthy persons . The statistical analyses revealed a significant decrease (p<0.05) in the concentration of hemoglobin (Hb), packed cell volume (PCV) , platelet count, and the total leucocytes count in the patients with CRF compared with the control group . The differential leucocytes count exhibited a significant increase in the percentage of neutrophils, monocytes and eosinophils ; while the percentage of lymphocytes decreased significantly (p<0.05) in the patients group The biochemical investigations revealed a significant increase (p<0.05) in the concentration of serum triglycerides and the activity of alkaline phosphatase (ALP) , and a significant decrease (P<0.05) in the concentration of serum high density lipoproteins (HDL) and the activity of aspartate & alanine transaminases (AST & ALT) in the CRF patients by comparing with control group .
In summary, the current study came to that chronic renal failure had affected physiological and biochemical parameters under study .

The effect of methods and spacings of planting on total yield of plant calendula officials

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 45-48

A factorial experiment with 12C131) of four replication was carried out iof determine
the influence of methods and spacings of planting on titalxield of flowers plant calendula officinals . methods of planting were furrows a part and rows a part spacings between plants were 10 , 20 and 30 cm . for both methods planting on furrows gave significant in crease in each of number of flower in fresh wt. fresh and dry yield perplant , percent of dry/ fresh wt. of flowers , number of flowers / plant , and fresh yield kg / ha. Mean while narrower spacings (10cm) between , plant produced higher number of flower in a fresh wt , fresh and dry yield of flowers kg / ha planting on furrows with (30 cm) between plants gave higher fresh and dry wt. flowers and higher number of flowers / plant . planting on furrows or rows of 10 cm between plants produced higher dry yield of flowers.

Effect of some products of honey bees in hydatid cysts and some hematological and immunological parameters in Balb/c mice

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 49-64

This study was conducted for the period from October 2009 until October 2010 ,which aimed to assess the effect of some products of bee honey(natural honey and royal jelly ) in hydatid cysts and some hematological and immunological parameters and find out the effectiveness of these products as immunomodifiers through study of the differential counts of white blood cells and the level of immunoglobulinsin Balb /c mice which infected with protoscolices of Echinococcus granulosus Parasite
The results of hydatid cysts counting showed after three months of the initial infection with protoscolices that the outputs of honey bee (natural honey and royal jelly ) very effective in reducing the number of in hydatid cysts in white mice ,where doses achieved (200 mg/kg royal jelly and 640 mg/kg natural honey) the therapeutic efficiency(100 %)and (95.18 %),respectively.Also the results of examine of some of blood parameters in mice revealed that the royal jelly and bee honey effects of increase in hemoglobin (Hb and hematocrit (pcv) and the total number of white blood cells (Total WBCs ).In the same context the results of the differential count of white blood cells of white mice showed that the royal jelly as immunodifiers and natural bee honey have significant effect in increasing the percentage of monocytes and slight decreasing in the percentage of eosinophils .Results of the single radial immunodiffussion test to estimate the concentration of immunoglobulins in white mice serum that the royal jelly and bee honey have effective in raising the level of theimmunoglobulins IgA , IgG and IgM .

Physiological study of A.niger & Penicillium fungi that isolution from seed of Arachis hypogeae & evaluation of the efficiency the Pseudomonas flourecens bacteria in inhibition the radial growth from the two fungi.

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 65-78

The study was conducted in the laboratories of Biology Department college of Sciences it is clarify the Physiological study of A.niger & Penicillium fungi that isolution from seed of Arachis hypogeae & evaluation of the efficiency the Pseudomonas flourecens bacteria in inhibition the radial growth from the two fungi. The laboratory experiments showed that the optimal pH for fungi growth in term of average dry weight for A. niger & Penicillium were pH6 with 0.35 gm and pH9 with 0.38 gm respectively but no growth about two fungi in pH3 . This study showed that efficiency from the two fungi growth in different of temperature level the optimal temp. for fungi growth in term of average dry weight for A. niger & Penicillium were 30c with 1.36 gm and 40 c with 1.38 gm respectively but the growth for A. niger in 20c & 40c with 0.23 gm and 0.28 gm respectively the growth for Penicillium in 20c &30c with 0.18 gm and 0.26 gm respectively also The laboratory experiments showed that efficiency A. niger & Penicillium growth in different of solt Nacl concentration , the optimal Nacl Con. for fungi growth in term of average dry weight for A. niger & Penicillium were 30 gm/l with 0.39gm &0.37gm respectively but the growth about two fungi in 10&20 gm/l with( 0.16 , 0.26) gm , (0.14 , 0.22 ) gm respectively This study showed that efficiency from the two fungi growth in different of clture media but the optimal media for fungi growth in term of average dry weight for A. niger & Penicillium were PDB with (1.48, 1.43)gm respectively but the growth about two fungi in YEB&NB with (0.33, 0.18)gm respectively for A. niger & (0.26 ,0.16) gm respectively for Penicillium . also The laboratory experiments proved the Pseudomonas flourecens bacteria had ahigh effect in inhibition the radial growth in concentration 2 g / L where the inhibition rate attained 100 % to the both two fungi .

Effect of the alcoholic extract of Achillea santolina on Reproductive organs of male in albino mice Mus musculus

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 79-87

This experiment was designed to test the effect of the alcoholic extract of Achillea santolina on fertility of male mice ,the treatment cause a number of pathological changes in the tissues of testes and epididymis . 1n this study was used (24) of the albino mice male of Balb/c breed , these mice were divided randomly in to a control group and three experiment groups were treated of concentration (200,250,300) mg/kg intrapertonealy for 25 daily.These study results were revealed the chemical analysis of the extract that contain secondary compound , alkaloid ,terpenoid , saponins , phenols and glycosides .there was a significant decrease (P<0.05) in avearage of treated animals body weight at all concentration as compared with the control group and there was significant differences between treated groups .the treatment with alcoholic extract of Achillea santolina plant has been resulted in many pathological changes in the germinal epithelium tissue that is lined testes as well as in epithelial cells which are lined epididymal tubules
It could be concluded that the alcoholic extract of Achillea santolina plant may have a pathological effect on testes ,epididymis in albino male mice which may influences the fertility.

Effect of NaCl on Callus growth of Catharanthus roseus and its vincristine and vinblastine alkaloids content.

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 88-92

An experiment was conducted at Plant Tissue Culture Laboratory / Horticulture Department / Agriculture College / Kufa University during 2008-2009 to study the effects of NaCl at concentrations ( 0, 25 , 50 or 75 mM ) concentrations on Catharanthus roseus cv. rosea callus growth initiated from in vitro multiple shoots, and its total indole alkaloids, vincristine and vinblastine contents.
Results showed significant differences in content of callus in total indole alkaloids, vincristine and vinblastine cultured at different NaCl salt concentrations in MS medium. NaCl treatments gave a significant increase in these parameters as compared with the control ( with out NaCl ). The highest amount of total indole alkaloids, vincristine and vinblastine (3.18 mg / gm callus dry weight, 5.12 µg / gm callus dry weight and 6.11 µg / gm callus dry weight ) respectively was found in the callus cultured in MS medium that supplied with 50 mM NaCl as compared with the lowest values(1.11 mg/ gm callus dry weight , 1.56 µg/ gm callus dry weight and 3.76 µg / gm callus dry weight ) in the control.

Detection of AmpC-β-Lactamases in Clinical Isolates of Pseudomonas aeruginosa in Najaf Hospitals

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 93-104

Pseudomonas aeruginosa is of the major causes of hospital acquired infection. The isolates have often been observed to display considerable resistance to multiple antibiotics, hence the aim of this study is to identify the dissemination of AmpC β-lactamase in clinical isolates of this bacteria. A total of 37 Ps. aeruginosa isolates were obtained from Najaf hospitals. The isolates were tested for their antibiotic resistance against 16 types of antibiotics. All the isolates were found to be resistant to a minimum of 3 classes of antibiotics, hence, the isolates were considered multidrug resistant. The primary antibiotic susceptibility test revealed that 10 (27%) isolates were cefoxitin resistant. These isolates were tested for their ability to produce AmpC β-lactamases using two methods, AmpC disk and modified three dimensional, also examined for the presence of blaAmpC gene. The AmpC enzymes production was confirmed in only 4 isolates. These isolates were examined for the presence of some extended-spectrum β-lactamases genes. Results revealed that 1 isolate carried blaSHV, 1 isolate carried blaSHV, blaTEM and blaCTX-M and 1 isolate carried blaSHV and blaCTX-M. According to the results carried out in this study, the AmpC producing Ps. aeruginosa represent a serious therapeutic challenge.

The effect of extracts and it's powdery in pathogenic fungi Fusarium oxysporum f.sp. lycopersesici and protect tomato plant from it infecation

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 137-147

This study aimed to test the effect of aqueous extracts of peel fruits of oak Quercus alba peel the fruits of pomegranate Punica granatum and the fruits of Citrullus colocynthis on growth and pathogenicity pathogenic fungus. Fusarium oxysporum f.sp.lycopersici The results of laboratory tests when the disclosure of chemical compounds effective in the water extracts studied the presence of most of the active substances in the extract of peel fruits of oak and influential in the growth of pathogenic fungus. The results showed the effectiveness of all the aqueous extracts used in the inhibition of fungal growth F.O.L where he excelled extract the rest of the in the inhibition of pathogenic fungus above when using the drilling wells with an average distance of inhibition 1.30 cm at concentrations of 10 and 20% compared with control treatment, which amounted to 0.00 cm in addition to the effectiveness of the aqueous extract of boiled peel oak in the inhibition of cumulative growth of pathogenic fungi, with total national average of 0.50 cm at concentrations of 5 , 10, 20% compared to the treatment of comparison, which amounted to 6.50 cm. while the aqueous extract of boiled peel pomegranates and the fruits of bitter melon is less effective in the inhibition of colony of F.O.L. It also achieved the aqueous extract of cold cobalt oak significant advantage in inhibiting the growth of pathogenic fungus as the growth rate the country 1.00 and 1.33 cm at concentrations of 2.5 and 5% respectively. It was found that the aqueous extract of the peel oak gave the highest rates of reduction in the rate of dry weight of pathogenic fungi at concentrations of 5 and 10%, which amounted to 0.00 gm for both concentrations compared to the treatment of comparison, which amounted to 6.16 gm. The regarding Added powder fruits Oak different concentrations in different soil and its impact on seed germination and seedling growth of tomato against pathogenic fungus F.O.L.were treated (Sterlezation soil+ 2.5% powder cobalt oak + fungus F.O.L) the best treatment to increase the percentage of seed germination, which amounted to 72% compared to the Pferoqat significant for my treatment comparison (Sterlezation soil only) and (Non sterlezation soil), who reached the 60 and 56%, respectively, also worked the same treatment to increase the percentage of seedlings of sound, which amounted to 62.33% and therefore have the same treatment to reduce the proportion of seed rotting and seedling infected and who reached the 28 and 9.66% and thereby protect tomato plants from infection .

Effect Of Ecological Pollutants on Vital Parameter Chlorophyll (A ,B ) & Some Anatomical And Morphological Parameters Of Conocarpus ,Vitis

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 159-175

The aim of this research was to assess the environmental pollution on the areas as aresult from different resources of polluted parameters vital effective chlorophyll ( A,B), anatomical and morphological features on leaves of ( Vitis vinifera ) and (Conocarpus sp.)plant . on three different positions in the Najaf governorate , (acontrol site Alb0 –Mathye ) which are very far from polluted resources .

A Systematic Numerically Study For some Species ofThe Family Apocynaceae inIraq.

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 176-189

The study had completed in the lab of sciences' of Kufa university ,depend of morphology, anatomy and pollen grain for of some species for Apocynaceae family in Iraq which be taken from AL-Zarkany(2004)Study .The treatment for character species Numbering and drawing .Polygonats for all species and numbering,similary percentage among and Agglomerative method diagram The results ensure for character .The morphology, anatomy ,and pollen grain which used in seprate .For some species of Apocynaceae.

Study effect the hot water exctract Alhagi maoururm to treatment mouse infect with Entamoeba histolytica

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 199-202

The present study aimed to investigate the possibility of the use of aqueous extract warm the roots of a plant Acol Alhagi maoururm in the treatment of mice experimentally infected parasite Entamoeba histolytica and three concentrations (250,500,1000) mg / kg and see how effective they are compared with the drug Metronidazole, which is used as a drug approved for the treatment of dysentery amebic results were that the aqueous extract warm the roots of a plant Acol more efficient in getting rid of the parasite and thus address the mice and get rid of the symptoms of the disease where it was to get rid of the parasite after three days at a concentration of 1000 mg / kg, and after four days at a concentration of 500 mg / kg, and after six days at a concentration of 250 mg / kg, while the results in the drug Metronidazole after the third day at a concentration of 1000 mg / kg, and after the fifth day at a concentration of 500 mg / kg, and after the seventh day at a concentration of 250 mg / kg.

Effect of Petrolium ether extract of Carrot Plant Seeds Dacus carota L. as antifertility on Female White rats

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 210-222

This study was conducted to recognize the effect of of petroleum ether extract of Dacus carota L. as antifertility to female white ( Rattus ratus), The study was performed in the animal Laboratory of Biology department in the Science Collage / University of Kufa throughout the period from (25/9/2010) to (20/3/2011) , in this study female white(Rats) were usedincluding (76) rats , the age of these females wereabout (8-12) weaks , here Suplemented by different concentrations (50 , 100 , 200) mg / kg of extract for three period of (10 , 15 , 20) days .The results of this study were revealed the followings :
• Asignificant decrease (P<0.05) in the weights of Reproductive organs (ovaries) of female white rats at all concentration (50 , 100 , 200) mg / kg and periods (10 , 15 , 20) days was highy effective in reducing the ovarian weight in The concentration (200) mg / kg and the period of (20) day comparing with the control group and other concentrations and periods .
.• Asignificant increase (P<0.05) in the weight of Uterus at all concentrations(50 , 100 , 200) mg / kg and all period of (10 , 15 , 20) day The concentration (200) mg / kg and the period of (20) days was highy effective than other concentrations and the periods increment of Uterine Weight
• Asignificant decrease (P<0.05) in the hormone levels : Follicle stimulating hormone (FSH) and Luteinizing hormone (LH) and Asignificant increase (P<0.05) in the Estradiol hormone level and the concentration (200) mg / kg and period of (20) day was highy significant comparing with the control group and other concentrations.
• The histological study was showed asignificant decrease(P<0.05) in the numbers of Primary , Secondary ovarian follicles and Graffian follicles and this study was showed Asignificant increase (P<0.05) in thickness of Endometrium of Uterus of female white rats and this study showed that the large significant (P<0.05) was in the concentration (200) mg / kg and period of (20) day comparing with the control group .• The results of female fertility have not been showen presence of sperm in vaginal smear unless beyond aperiod of time and pregnancy period would be late through dialy watchingfor (60) day. Aclear decrease in percent of pregnancy and in numbers of embryos of female White rats that treated by the concentration(50 , 100 , 200) mg / kg comparing with the control group .
The study was Concluded that Petrolium ether of Carrot Seed act as antifertility by their effect on embryos numbers , pregnancy percent and hormonal leves (FSH , LH and Estradiol) .

Test the efficiency of Bacillus circulans in protecting wheat plant from infection by Pythium aphanidermatu

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 223-230

Abstract A series of laboratory and field experiments to test the efficiency of the bacteria Bacillus circulans in controlling wheat root rot disease caused by Pythium aphanidermatum . The results showed that seed of wheat treated with B.circulans gave a high seed germination percentage reached 91.3% with the lowest number of seedling death of 5.1% for control treatment (wheat seed alone), which amounted to 80.3% and 12% respectively. The results of this experiment confirmed the ability of bacteria to provide high protection to plant wheat against the influence of P. aphanidermatum where treatment bacteria gave the highest percentage of germination of seeds of wheat planted wheat planted Petrb contaminated with pathogenic fungi, which amounted to 86% while the percentage of germination in the control treatment (62%) and the plant death percentage was at lowest level at same treatment which were 17.96% compare with control ( 58.03%) The results showed in significant increase in the growth indicators (plant height , tellaring number ,fresh weight and dry shoots and roots weight ) as well as production parameter including number and length of spike, grain / spike and weight of 1000 grains.

The Effect Of Sodium Fluoride at Different concentration On Some Organs ( The Liver, Spleen And female Reproductive System ) Of Newhampshire Hens. Gross – Pathological Study.

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 245-252

Fifteen newhampshire hens at nine month old used to determine the effect of different levels of sodium fluoride (150 PPM , 300PPM). The period of experiment reached two months. The first group administrated (150PPM) sodium fluoride with ration / daily, while the second treated group, administrated (300PPM) sodium fluoride with ration / daily. Each treated groups revealed a variety of relative pathological changes which characterized in the first treated grou by mild pathological changes in the spleen, liver, ovaries and ovi-duct. Which involvement enlargement of these organ. While morphological and pathological changes which occurs in second treated group these pathological alterations which included, sever enlargement of liver and spleen, the liver was pale-yellowish in appearance and ecteric , paeticael haemorrhage was distributed on the lower surfaces of right and left lobes. Also, the spleen was enlarged, congested and dark – reddish in the colour. The ovaries in second treated group were appeared congestion in the ovarian follicles. And some of ovarian follicles was atrophied, interstitial oedema was found among ovarian follicles and fibrosis occur in the infundibulum and magnum led to retention of eggs

Estimation of the Level of Total Carbonyl and Malondialdehyde in Thalassemic Patients and Study their Correlation with Iron Status Parameters

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 253-259

In the present study, an attempt is carried out to estimate the degree of iron overload in thalassemia major patients in addition to the measurement of lipid peroxidation end product, malondialdehyde (MDA), and protein end product, total carbonyl. The level of these compounds will indicate the risk of tissue damage caused by oxidative stress and iron overload in thalassemia patients.
One hundred and eighteen Arabic Iraqi patients with major thalassemia were participated in the present study. Their age range was 4-12 years old. Thirty apparently healthy children were selected as control group.Serum levels of iron and total Iron Binding Capacity (TIBC) were measured spectrophotometrically while unsaturated iron-binding capacity (UIBC), estimated total iron body stores (ETIBS), transferrrin saturation percentage (TS%) and transferrin concentration were calculated mathematically. Serum ferritin, MDA, and total carbonyl were measured using ELISA technique.
The results showed significant increase (p<0.05) in all iron indices of thalassemic patients in comparing with healthy control group except TIBC, UIBC, and transferrin concentrations, which decreased significantly (p<0.05) in those patients in comparing with control group. Serum total carbonyl and MDA are increased significantly in thalassemia patients as compared with control group.
It can be concluded that Iraqi thalassemic patients are at high risk for iron overload and iron-induced toxicities. Protein carbonyls and MDA are elevated in those patients. These patients are prone to tissue injury caused by oxidative stress. There is no statistically significant correlation noticed between each serum MDA and total carbonyl with every component of iron status. The mechanism of action of iron in the body is differ from that of the oxidation products of proteins and lipids (total carbonyl and MDA, respectively).

The determination of total mercury in blood and urine samples collected from people worker in and living beside the Al-Furat Company for Industrial Chemistry- Saddat Al-Hindia/ Iraq

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 260-265

Mercury toxicity can be predicted by the detection of elevated concentrations of mercury in blood and urine samples. A cross sectional study was conducted to evaluate the mercury levels in blood and urine samples of twenty five persons of workers from Al-Furat Company and people living beside. The persons enrolled were divided into five groups according to their potential mercury contact. All samples were digested and analyzed by Cold Vapor Atomic Absorption Spectrophotometer (CVAAS). Two factors were considered in the present study: age and duration of potential exposure to mercury. The results showed that the group one revealed higher mercury concentration in blood and urine (mean=0.05394 µg/ml, p-value ≥0.001 and mean= 247.6178, p-value ≥0.05respectivlly) in comparison with all other groups. Regarding the age, the people is divided into three age intervals, there is a positive correlation between age and mercury in blood, the age interval more than forty years revealed high mercury level in blood (mean=0.02691 µg/ml) in comparison with other two intervals, while the youngest people (less than thirty years), excrete higher mercury concentrations in urine (mean=127.702 µg/ml) than older. Moreover, the group of long period of exposure (more than twenty years) revealed an increased mercury levels in blood (mean=0.02958 µg/ml). However, there is diminishing in mercury concentrations in urine in the interval more than twenty years of exposure (16.7155 µg/ml).

Evaluation the relation between the nutrient deficiency and oral manifestation in Najaf city.

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 266-270

The relationship between nutrition and oral health is multifaceted. Nutrition has both local and systemic impacts on the oral cavity. Nutrient deficiency may result in oral symptomatology. There are many factors effected the nutrient as age, sex, and pregnancy. Clinical manifestations of nutrient deficiencies can have a significant impact on the function of the oral cavity. Functional properties of the oral cavity include taste, salivation, mastication, and swallowing food. The study was carried out on 150 cases from hospitals and special clinics during period from December 2010 to May 2011 in Al – Najaf city. All patients had been involved in this study from both sexes, the age between 12 – 65 years (120 females and 30 males), females divided in pregnant women 50, the ages between 15 – 38 years and non-pregnant women 70, and the ages between 12 – 65 years. The mouth is frequently the mirror of the body involved in conditions that affect the skin or other multiorgan diseases. In many instances, oral involvement precedes the appearance of other symptoms or lesions at other locations. Dentists and health professionals must recognize the manifestations of these deficiencies consider their causes and provide early treatment to prevent further compromise in nutrition status and to promote optimal nutrition, oral and overall health

study for intestinal parasites among children in AL-mahmoudyia area / Baghdad province

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 271-274

This study was carried out to find the infection with intestinal protozoa such as (Entamoeba histolytioca , Giardia lamblia) and Enterobius vermicularis in children as diarrhea causative in AL-mahmmodia public hospital for a period from the beginning of June 2010 to the end of August 2010 .out of (332) stool samples there were (296) positive sample which represented (57.8%) these sample were examined by using direct normal saline method , Iodin solution and sedimentation method . the percentage of infection with intestinal parasite as followed Entamoeba histoloytica (41%) , Giardia lamblia (34%) ,and Enterobius vermicularis (25%) . These results considered high in compression with other studies carried out in Iraq .The infection percentage in male was (57%) which in female was (43%) .July was the highest month in compression with may , June –respectively .

Detection of Plasmid Profile coding Antibiotic Resistance of Klebsiella spp. Isolated From Different Infection

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 275-281

One hundred and fifty five of Gram-negative-lactose fermented bacteria grown on MacConkey agar were collected from burns, wounds, UTI and RTI in two hospitals of Najaf province. The isolates were identified according to cultural characteristics and biochemical activities. 43 (27.7%) isolates were Klebsiella spp. represented by 35 (81.3%) were K. pneumoniae subsp. aerogenes, 4 (9.3%) was Klebsiella pneumoniae subsp. pneumoniae, 2 (4.6%) was Klebsiella oxytoca, as well as one isolate for each of K. pneumoniae subsp. ozaenae and K. pneumoniae subsp. Rhinoscleromatis. Plasmid profiles of all Klebsielae isolates were studied and the results have revealed that 37 (86%) isolates had two distinct plasmid bands.

Congenital malformations and determined rate in Diwaniyah city.

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 282-289

This study analysis of congenital malformation among birth in Diwaniyah city for period extended from June-August in 2008.Itwas 3400 liveborn babies ,42(1.24%) infants were diagnosed with congenital malformations and the percentage of congenital malformations was 1.87%,1.17% and0.87% for three months respectively. The results showed that congenital malformations increased with infants had type O+ group and parents with first degree in consanguinity.

Some Histological description of lingual papillae in the Millivora capenesis tongue Tawfeeq jawad ali

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 290-295

The present study was conducted on three tongues of Millivora capenesis animal. Histologically, the dorsal surface was covered by keratinized stratified squamous epithelium ,while the ventral surface which covered via non-keratinized stratified squamous epithelium . Five types (filliform,conical,fungi form,circumvallate and foliate)papillae were project and distributed on dorsal and lateral surface.The fillifom was similarly to threads or like hairs ,while conical was cylindrical shaped. The fungi _form papillae like the mashroom ,the wall contain taste buds ,but the circumvallates papillae circular in shape and embedded in the epithelium, the foliate papillae was similar ridges, both circumvallate and foliate papillae were contain taste buds.

Possible role of some aspects of immune response in aborted women infected with Toxoplasma gondii

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 296-302

The immune response that was induced by Toxoplasma gondii infection was found to be protective to mother, but it was deleterious to the fetus, i. e. the infection induced an immunopathological state in the fetus. The induction of high levels of IFN – γ and IL – 10 due to T. gondii infection may exert an inhibitory effect on serodiagnosis (inhibition the synthesis of progesterone and estradiol) which may explain their possible role in termination of pregnancy. The mechanisms of the other factors may be also induced after T. gondii infection.

Comparative study to Some Microbial and Physiochemical parameters between Al-Kufa and Al-Manathera water before and after treatment

Magazine of Al-Kufa University for Biology, 2012, Volume 4, Issue 1, Pages 303-313

The present study aimed to compare some microbial and Physiochemical parameters of Al-Kufa and Al-Manathera water before treatment (river water) and after treatment , the results revealed that there was significant increase (p=0.0082) in total available count (TVC) of Al-Kufa water before and after treatment since it was (25.666 cfu/ml and 0 cfu/ml) respectively . Moreover there was significant increase (p=0.0020) in total available count of Al-Manathera water before and after treatment since it was ( 32.666 cfu/ml and 0 cfu/ml ) respectively . The results of present study indicated that there was significant increase( p=0.0028 ) in total coliform count (TCC) in Al-Kufa water before and after treatment since it was ( 16.666 cfu/ml and 0 cfu/ml ) respectively and there was significant increase ( p=0.0075 ) in total coliform count in Al-Manathera water before and after treatment since it was (21.33 cfu/ml and 0 cfu/ml) respectively , and the results of this study improved that there was no growth for Salmonella.spp and V.cholera in Al-Kufa and Al-Manathera water after treatment.
Also the present results indicate that there was significant increase( p= <0.0001 ) in total dissolved solids (TDS) in Al-Kufa water before and after treatment when it was ( 1188.3 ml/l and 854.67 ml/l) respectively , and there was significant increase ( p=<0.0001 ) to total dissolved solids in Al-Manathera water before and after treatment when it was ( 854.67 ml/l and 869.00 ml/l ) respectively , also the study improved that there was significant increase( p=<0.0001) in turbidity in Al-Kufa water before and after treatment when it was ( 34.667 ftu and 3.3133 ftu) respectively , and there was significant increase ( p=<0.0001 ) in turbidity in Al-Manathera water before and after treatment since it was ( 38.000 ftu and 3.2567 ftu ) respectively , and there was no significant increase between Al-Kufa and Al-Manathera water in turbidity after treatment , also the results demonstrated that was no significant increase between Al-Kufa and Al-Manathera water in acidity (PH) levels before and after treatment.