Isolation and identification the fungi associated with broad bean aphid and testing their pathogenicity and using some of them as a biological agents against the insect

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 1-12

This study aimed to isolate and identify the fungi associated with the broad bean black aphid Aphis fabae Scopolli . The pathogenicity of these fungi and the biological effect of some of them on the different insect instars were also studied . Nine species of fungi were isolated , they were Alternaria alternata, A. chlamydospora, A. niger, Beauveria bassiana, Cladosporium oxysporum ,Penicillium chrysogenum, P. compactum , Ulocladium atrum and sterile white mycelium. P. chrysogenum and A. Chlamydospora gave percent of high occurrence reached to 24.6 and 18.4 % respectively , while A. niger and white mycelium gave a less percentage of occurrence which were 3.07 and 1.5% respectively. The pathogenicity test indicated that B. bassiana and C. oxysporum were entomopathogenic fungi compared with the other isolated fungi , the mortality reached to 54.8 and 44.5% for tow fungi respectively.The fungal suspensions of these fungi in concentration of 1x 108 spores/ml led to kill the insect at mortality of 39.2 and 35.68% respectively. On the other hand , the exudates of these tow fungi in concentration of 100% revealed a high percent of mortality in nymphs and adults of aphid reached to 52.17 and 54.10 % respectively, while C. oxysporum exudate led to kill the nymphs and adults in percent of 55.5 and 50.6 % respectively .
This paper is a part of Msc. Theses of the third author.

Role The FertiCult Flushing Medium in Sperm Parameters Activation In Vitro of Infertile Patients Suffering from Moderate Asthenospermia

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 12-20

This study was included examination and activation of 34 samples for asthenozoospermic men.The research was aimed to improvement of infertility capacity and activation sperm parameters in vitro by using FertiCult flushing medium ,three techniques (swim up-centrifugation wash-out-mixing after washing and centrifugation).The result was revealed significant increase (p<0.05) of sperm motility percent ,grade of sperm activatiy and sperm motility index so significant decrease (p<0.05) of sperm concentration ,non normal sperm morphology percent and leukocytes concentration after using the medium and swim up in 60 min .In conclusion,the FertiCult flushing medium influence of the result after activation and increase of improvement the chance in vitro fertilization.

Measurement of some immunological sides for pations with chronic lymphoid leukemia

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 21-29

The present study was conducted to evaluate some immunological parameters in acute lymphoblastic leukemic patients , which it : Total and Differential leukocytes , adenosine deaminase ( ADA) specific activity , mean in interferone gamma (IFN-γ) .Thirty patients were randomly selected from AL-Sadur hospital in Najaf city , In addition thirty healthy subjects enrolled as a control group. However , the pretreatment group fifteen patients and resistant group fifteen patients . The results of this study confirmed significant increase in the total count of white blood cell in both group of patients ( pretreatment group and posttreatment group ) in comparison with control group (8000 ± 2100 , 90000 ± 6400 , 13100 ± 4500) .The leukemic patients showed significantly reduced levels of plasma adenosine deaminase activity which was (0.90 ± 0.04) in pretreatment group and (0.65 ± 0.01 ) in resistance group , in comparison with those of the control group (1.45 ± 0.3) , at the same time the results showed reduced mean of IFN-γ in the pretreatment group (1.66± 0.26) and in resistance group (1.34 ± 0.20) in comparison with those of the control group) 2.68 ± 0.46) .

Using of some cold water plant extracts of sativus Crocus, Curcuma longa and Matricaria chamomilla in staining of Cestods Isolation from Streptopelia decaocto.

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 30-36

This study was carried out for testing the efficient of cold water plant extracts of sativus Crocus, Curcuma longa and Matricaria chamomilla in staining the tegument of Cestods Isolation from Streptopelia decaocto.The results of this study reported to the cold water extracts of Crocus sativus and Curcum longa were the best in staining of helminthes ,where their the Matricaria chamomilla doesn’t give positive results in staining.

A study of the effect of exposure time of Ultra violet radiation on the Female Albino Mice Fertility

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 37-45

This study was performed in the physiological Laboratory in the Science College / University of Kufa to evaluate of the effect of Ultra violet radiation on Swiss Female Albino Mice ( Mus musculus ) of ( Bulb / c ) strain including (12) mice , these females were divided into three groups according to different periods (24 , 48 , 72) hour and compared with control group This results were revealed a significant decrease ( p> 0.05 ) in diameters of ovaries and numbers of Primary & Secondary ovarian follicles and numbers of Graffian follicles of female mice that treated with Ultra violet radiation and compared with control group .

Evaluate the efficiency of Biocides Thurine wp the protect vitality of the wheat grains contaminated with Aspergillus flavus stored and their ability to germination

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 46-58

of the wheat grains contaminated with fungi Aspergillus flavus stored and their ability to germinate, was isolated from many fungi associated with grains of wheat, including fungi and A.flavus and A.niger and A.terreus and Penicillium spp and Rhizpusspp The fungus most frequently A.flavus reaching 98% compared to other fungi. And the results indicated that A.flavus showed sensitivity to all concentrations of biocidal tested and the rates varied among themselves morally, as the biocide user, and reached the highest inhibition at the concentration 1 g / l to 100% , 92% at the concentration 0.5 g / l , In addition, the results of statistical analysis to a significant difference in the numbers of bacteria after of seeds, having already overtaken the Treatment of biocide concentration of 10 g / kg seeds contaminated with A.flavus in the numbers of bacteria, amounting to 2.64 × 1012 colony / cloud of the A.flavus in when they arrived, their numbers 0.98 × 1012 configuration console colony / g of the seeds when treated with biocide concentration 5 g / kg seeds. In addition, the results show the efficiency of different concentrations of the biocide is vital in reducing the incidence of fungi A.flavus grain the transaction after the expiration of the storage period of three months, as the percentage of infection fungi 20 , 0% for concentrations of 5 and 10 g / kg, respectively. Differed significantly on the treatment of comparison the rate was 90% Furthermore, it indicated that results of the evaluation activity and the effectiveness of biocidal Thurine wp covered with grains of wheat in the soil after three months of storage on germination percentage after fast A.flavus in the vitality of seeds of wheat stored, which dropped to 39 % while it rose to 98 , 100% in grain treated with biocide concentration of 5 and 10 g / kg seed, respectively. While rates have decreased the proportion of the death of seedling to 2 , 0% in cereals treated with biocide concentration of 5 and 10 g / kg seed in comparison with control, the percentage of the death of seedling to 61%. The indicators of growth and production will assure a significant difference in the rate of increase in plant wheat treatment concentration 5 and 10 g / kg of biocidal. It was the highest height of the plant to concentrate 10 g / kg seed, as was 21.53 cm while the average plant height in the treatment of comparison 12.33 cm The concentration 5 g / kg seed rate of plant height

Selection of Suitable culture medium with the use of wheat and corn plants which irrigated by extract of aqueous manure.

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 67-76

An experiment was conducted in the laboratories and green house of Biology Dept. Education College for Girls Univ. of Kufa for 2009 –2010 agricultural season , included the test of eleven culture media which were (non saline soil as "control" , saline soil, cotton , sand , gravel , small stone , sawdust , industrial cork , stiff paper , peat moss and rise shells ) in order to choose the best medium through growth parameter response of wheat and corn plants as ( test plants ) to study soilless culture subject by using manure aqueous extract in plants irrigation .
Results showed that, sand and sawdust media were best culture media among the different cultures in this study , due to their highest germination percentage 90% and 92% , respectively as compared with control which was 75% . Results also revealed that the highest plants growth parameters ( shoot and root length , leaf area , wet and dry weight ) were achieved from the sand media as compared with sawdust response for the same parameters .

Isolation and Identification of the Fusarium species in three soils types and their pathogenicity on radish seeds germination in laboratory

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 85-98

The study included isolation and identification of Fusarium Species from three soil types by dilutions plate method and plant debris in soil , the soil location were desert soil beside Najaf - Karbala road , Alkufa riverside soil and Hurriya rice field .The soil samples and plant debries were taken from half of July ,October 2009 and January and April 2010 . In this study 13 species of Fusarium were isolated , the study showed that the following species : F.konzum , F.circinatum , F.phyllophilum , F.pseudonygamai , F.beomiforme , F.hostae , F.dlamini , F.lateritium F.nelsonii and F.denticulatum . were recorded as first time in Iraq . The results revealed that the high frequency 6.6 % of the Fusarium species was in desert soil during the summer season . F.konzum was isolated 100 % frequency from the desert soil in July 2009 compared with other species . The pathogenecity tests to radish seeds on P.D.A. showed that the F.beomiforme killed all the tested seeds while seed germination in F.hostae and F.semitectum treatments were 94.67 and 92 % respectively . F.lateritium non significantly decreased the Shoot length (12.2 mm) of radish compared with control treatment (12.4 mm) while the F.nelsonii (isolate B) incrased the shoot length (12.63mm) compared with control that rate (12.oo mm) . F.denticulatum produced Aflatoxins while the other species gave negative result .

Isolation and Identification of the Fusarium species in three soils types and their pathogenicity on radish seeds germination in laboratory

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 85-98

The study included isolation and identification of Fusarium Species from three soil types by dilutions plate method and plant debris in soil , the soil location were desert soil beside Najaf - Karbala road , Alkufa riverside soil and Hurriya rice field .The soil samples and plant debries were taken from half of July ,October 2009 and January and April 2010 . In this study 13 species of Fusarium were isolated , the study showed that the following species : F.konzum , F.circinatum , F.phyllophilum , F.pseudonygamai , F.beomiforme , F.hostae , F.dlamini , F.lateritium F.nelsonii and F.denticulatum . were recorded as first time in Iraq . The results revealed that the high frequency 6.6 % of the Fusarium species was in desert soil during the summer season . F.konzum was isolated 100 % frequency from the desert soil in July 2009 compared with other species . The pathogenecity tests to radish seeds on P.D.A. showed that the F.beomiforme killed all the tested seeds while seed germination in F.hostae and F.semitectum treatments were 94.67 and 92 % respectively . F.lateritium non significantly decreased the Shoot length (12.2 mm) of radish compared with control treatment (12.4 mm) while the F.nelsonii (isolate B) incrased the shoot length (12.63mm) compared with control that rate (12.oo mm) . F.denticulatum produced Aflatoxins while the other species gave negative result .

Isolation and Identification of the Fusarium species in three soils types and their pathogenicity on radish seeds germination in laboratory

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 85-98

The study included isolation and identification of Fusarium Species from three soil types by dilutions plate method and plant debris in soil , the soil location were desert soil beside Najaf - Karbala road , Alkufa riverside soil and Hurriya rice field .The soil samples and plant debries were taken from half of July ,October 2009 and January and April 2010 . In this study 13 species of Fusarium were isolated , the study showed that the following species : F.konzum , F.circinatum , F.phyllophilum , F.pseudonygamai , F.beomiforme , F.hostae , F.dlamini , F.lateritium F.nelsonii and F.denticulatum . were recorded as first time in Iraq . The results revealed that the high frequency 6.6 % of the Fusarium species was in desert soil during the summer season . F.konzum was isolated 100 % frequency from the desert soil in July 2009 compared with other species . The pathogenecity tests to radish seeds on P.D.A. showed that the F.beomiforme killed all the tested seeds while seed germination in F.hostae and F.semitectum treatments were 94.67 and 92 % respectively . F.lateritium non significantly decreased the Shoot length (12.2 mm) of radish compared with control treatment (12.4 mm) while the F.nelsonii (isolate B) incrased the shoot length (12.63mm) compared with control that rate (12.oo mm) . F.denticulatum produced Aflatoxins while the other species gave negative result .

Isolation and Identification of the Fusarium species in three soils types and their pathogenicity on radish seeds germination in laboratory

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 85-98

The study included isolation and identification of Fusarium Species from three soil types by dilutions plate method and plant debris in soil , the soil location were desert soil beside Najaf - Karbala road , Alkufa riverside soil and Hurriya rice field .The soil samples and plant debries were taken from half of July ,October 2009 and January and April 2010 . In this study 13 species of Fusarium were isolated , the study showed that the following species : F.konzum , F.circinatum , F.phyllophilum , F.pseudonygamai , F.beomiforme , F.hostae , F.dlamini , F.lateritium F.nelsonii and F.denticulatum . were recorded as first time in Iraq . The results revealed that the high frequency 6.6 % of the Fusarium species was in desert soil during the summer season . F.konzum was isolated 100 % frequency from the desert soil in July 2009 compared with other species . The pathogenecity tests to radish seeds on P.D.A. showed that the F.beomiforme killed all the tested seeds while seed germination in F.hostae and F.semitectum treatments were 94.67 and 92 % respectively . F.lateritium non significantly decreased the Shoot length (12.2 mm) of radish compared with control treatment (12.4 mm) while the F.nelsonii (isolate B) incrased the shoot length (12.63mm) compared with control that rate (12.oo mm) . F.denticulatum produced Aflatoxins while the other species gave negative result .

Histological Study of skin in local embryo chickens at seven days age by using different stains methods

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 99-107

This study was investigated in each lab of histological preparations of Artistry technical institute / Kufa and Al-Sadr hospital in Najaf. The period of histological study is from 10/9/2009 to 10/1/2010, the aim was studying histological the skin in local embryo chick at seven days by using different stains methods and measurement of the epidermis and dermis thick in feather bud and interbud regions , twenty five eggs of native chicken were collected,and used natural incubation was.after seven days, Four eggs were hatched for Samples of embryos skin’s were removed and subjected to histological procedures,used three method of stain: Harris Haematoxylin – Eosin, PeriodicAcid-SchiffandVerhoeff-Van Gieson The results of present histological study demonstrate that the skin of seventh days stage differentiated to tow layers: Upper layer is epidermis, which arranged in two rows of cells, the outer row is the periderm and the inner row is the basal layer which thickened in pterylae to form epidermal placode. The lower layer is dermis which formed from loose mesenchymal tissue, it contains mesenchymal cells and fibers. In this stage, dermal condensation can be seen under epidermal placodes that give short feather buds and lightly long buds in which, invaginated in base of its epidermis that assumed to be present in the following stage.

Acomparative anatomical study of some species for papilionidae ( leguminosae ) in Iraq .

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 108-126

The present study included acomparative anatomical study of leaf and cross section of stem, petiole and blades in some genera of papilionoidae in Iraq. The present study included the species Glycyrrhiza glabra L. , Lablab purpureus (L.) Sweet, Lathyrus annuus L., Trifolium alexandrinum L. , Vigna radiate (L.) Wilczek and species V. urguiculata (L.) Walp. Anatomical study of blades, petioles and stems showed diagnostic value to differentiate the species from other under study , such as absent distribution of the stomata on adaxial surface to the leaf of Lablab sp., distribution secretory chanals tanine whithen palaside layer in the cross section of leaf and different parts of stem of Glycyrrhiza sp., and the winged stem con be used also as diagnostic character to identify Lathyrus sp. , and other characters.

Changes in some physiological criterioi and relation with pations womens of polycystiv ovary syndrom

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 127-137

The present study was conclude in Al-Najaf Al-Ashraf fertility and steril Center in Al-Sadir teahing hospital during the period between 1/12/2008 to 1/1/2010 The study was carried out on 150 women that on polycystic ovarian syndrome compared with the control group that was carried out on 50 women . Their fertility have been confirmed and that they have no other disease like artery diesase, thyroid gland disease diabetes mellitus and blood pressure. Depending on the age of the women , the samples were divided into three groups, the first group(15-25) years , the second group (26-35) years and the third (36-45) years . Also they were divided depending on C-Reactive protein into two groups , the first group included 80 disease condition and the second group 50 normal condition .The study revealed a significant increase of total count of Leucocyte compared with the control group, the diffirentatial number of Leucocyte revealed significant increase of Neutrophille percentage , also the percentage of Monocyte increased and the CRP+ revealed more increase of leucocyte compared with the control group . The results revealed significant decrease (p<0.05) in hemoglobin concentration and significant increase (p<0.05)in total count of blood tablete compared with control group .

Diagnosis of causative bacteria of urinary tract infection to children delivery Al-Diwanyia in hospital

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 138-140

Afield study aimed to identify to know the causative aigent of urinary tract infection to children under 5 years . the study included ( 60 ) sample of urine from a chilled suffering from UTI in chilled and delivery AL-Diwanyia hospital during ( February 2010 to may 2010) .The result of the study revealed that E.Coli it was the more causative aigent of this study accounted 51,7% and Pseudomonas . Aerogenosia accounted 20%. another causative divided between many types of bacteria Klebsilla spp. 13,3 %. Staphylococcus . areause 8,3%.and Protues spp. 6,7%. .

Changes in some chymobiological criterioi in pations womens of polycystiv ovary syndrome

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 141-153

The present study was conclude in Al-Najaf Al-Ashraf fertility and steril Center in Al-Sadir teahing hospital during the period between 1/12/2008 to 1/1/2010 .The study was carried out on 150 women that on polycystic ovarian syndrome compared with the control group that was carried out on 50 women . Their fertility have been confirmed and that they have no other disease like artery diesase, thyroid gland disease diabetes mellitus and blood pressure.Depending on the age of the women , the samples were divided into three groups, the first group(15-25) years , the second group (26-35) years and the third (36-45) years .The results revealed a significant increase of low density lipoprotein (L D L ) concentration and there was no significant difference in high density lipoprotein (H D L) compared with the control group, also there was a significant increase (p<0.05) in the total cholesterol concentrations and trglycerides compared with the control group. No significant change appeared in Aspartate amino transferase enzyme (AST) concentration but it showed a significant increase (p<0.05) in Alanine amino transferase enzyme (ALT) concentration compared with the control group .There was no significant changes in the calcium concentration, but a significant increase (p<0.05) appeared in sodium concentration and a significant decrease (p<0.05) in the potassium concentration when compared with the control group .

Study of changes hormonal inblood serum of womens with who discontinue of oral contraceptives in city of Al-Najaf

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 154-166

The present study was concerned to investigate the disturbances that occurred in some hormonal and Criterions in blood serum of women who discontinue of oral contraceptives for different periods ranged from(6–30) month.The study was carried out in AL-Zahra hospital Laboratories for birth and children, in Najaf city ,within period from 1/8/2008 to 1/2/2009This study composed of (101) women ,were devided into two groups : The first group consisted of (83) women who were suffering from prolongation of the perios to be pregnant after the left using the oral contraceptives pills, although these women were previously fertile ,because each of them had born at least one baby before .The second group consist of (18) fertile women . who were never used any kind of contraceptives . All women ages ranged from 24–38 yearResults of all required tests were obtained from the first group compared with that results obtained from control groupStatistical analysis of results revealed that , there was significant decrease (P<0.05) in concentration of both follicle stimulating hormone (FSH),and Luteinizing hormone (LH) ,during follicular phase ,and this decreasing was significantly shown among all age groups ,which is higher decrease from third group (34–38),and from fourth stopping duration(25–30) month ; and the concentration of FSH & LH was reversely decreased where as the ages of women were increased. Otherwise .The study exhibited also a highly significant increase in concentration of both progesterone and estradiol hormones ,and this increasing was significantly shown among all age groups which is higher increase from third group (34–38) of women who were left using oral contraceptive pills ,indifferent periods which is higher increase from first stopping duration(6–12) month .Mean while ,there were no significant differences occurred in prolactin hormone level, in the same groups women and among all age groups also.

The Effect of Phosphate on Growth Pseudomonas aeruginosa In vitro

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 167-178

The aim of of this study was to determine the effect of phosphate compounds on the growth of Pseudomonas aeruginosa in vitro and in vivo. This bacteria has the ability to search for the non organic phosphate found in the tissue or in the culture media, this phosphate is very important for the metabolism and growth of this bacteria .
In vitro the study of phosphate concentration effect on bacterial growth in the liquid media reached an optimum of 13% while in the solid media it was 17%. The increase of phosphate concentration caused increase in the growth of this bacteria. This study showed the ability of their bacteria resistance for most antibiotic tested.

Prevalence of Oral Protozoa in Periodontitis and Gingivitis Patients Whose Attended to Clinics Periodontics , Dentistry CollegeBabylon Univ.

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 179-184

Trichomonas tenax, a commensal flagellated protozoan, inhabits in human oral cavity. This parasite is cosmopolitan and frequently found in patients with poor oral hygiene and advanced periodontal disease. By using wet mount smear and giemsa staining to detect the prevalence of oral protozoa in patients with oral diseases and a healthy control group.From October 2009 to April 2010, the subgingival dental plaques of 310 patients with gingivitis or periodontitis and 310 controls who attended to clinics periodontics , dentistry college- Babylon university. 64 (20.6 %) of patients were positive (40.2 % periodontitis ,14.2 % gingivitis) by using wet preparation and Giemsa staining.. The prevalence of oral Trichomonas tenax in our study (20.6%) and Entamoeba gingivalis was (42.9 %) was compatible with many other published reports which mostly has ranged from 12%-32%.The study revealed dependence between the frequency of occurrence of protozoa and the state of periodontitis.the age group (41-50)yr. Have high incidence of T.tenax compared with an other groups,as well as the males have high incidence(24.7 %) than females (16.8 %).

The relationship between tuberculosis and Diabetes Mellitus in patients

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 185-192

Diabetes Mellitus is associated with higer risk of lower respiratory tract infection including pulmonary tuberculosis with features which differ from that observed in the general non-diabetic population.To assess the effect of diabetes mellitus on the clinical ,bacteriological and radiological presentation of pulmonary tuberculosis diabetic population. This is a cross sectional study that enrolled 50 patients with diabetes mellitus and pulmonary tuberculosis . the patients included in this study had been randomly selected from those attending AL-Hilla Teaching Hospital in Babylon Province – iraq during the period between the 1st of May, 2006 to the 31st of May , 2007. All of the patients had been subjected to thorough physical examination and laboratory investigations .This study revealed male to femaIe ratio of 5:1. Sixteen percent of the sample ( 4 patients ) was of type 2 diabetes . mellitus. 56% of those with diabetes and pulmonary tuberculosis (14 patients) were diabetic for 0-10 years. 38 patients (76% of the sample ) had sputum smear positive , the remaining 12 patients (24 % of the sample) were smear negative / culture positive .Multiple lobe involvement was obvious in 64% of (32 patients ). lower lung field involvement had been detected in 32% of the sample ( 36 patients ) . Caviary lesions had been diagnosed in 30 patients (60% of the sample).This study revealed that diabetes modifies the clinical and radiological picture of pulmonary tuberculosis . Diabetic patients are at higher risk of recurrence and extensive pulmonary involvement . Tuberculosis is associated with poor glycemic control . Diabetes changes the male : female ratio toward the side of male predominance . Lower lung field involvement is more common in diabetics who had pulmonary tubrcu1osis.

Molecular Detection of AmpC Gene Encoding Antibiotic Resistance among Klebsiella spp. Isolated from Different Infections

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 193-200

One hundred and fifty five of Gram-negative-lactose fermented bacteria grown on MacConkey agar were collected from two main hospitals in Najaf province. The isolates were identified according to cultural characteristics and biochemical activities. The ability of Klebsiellae isolates to AmpC production were tested; the results have revealed that 9 (30%) isolates produce AmpC β-lactamase. PCR amplification results have shown that six Klebsiellae isolates possess AmpC β-lactamase gene.

Study of Effects of propolis on the some Biochemical parameters in broiler chickens.

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 201-205

Propolis (bee glue) is the resinous substance collected by bees from the leaf buds & bark of trees, especially poplar & conifer trees. Bees use the propolis along with bees wax to construct their hives. Propolis has properties, such as antibiotic activities, anti-inflammatory, anesthetic, Immunomodulatory, antioxidant, carcinostatic. The purpose of this study was to analyze some biochemical parameters of bee glue in drinking water of small chickens. The experiment included three groups : first group (6 chicks) was given the drink water added to alcoholic propolis in 20% for month twice daily. The second group (6 chicks) was given alcohol only at 20% while third group (6 chicks) was given drinking water only. The results showed no significant differences at (p<0.05) in the first group ( treated group) as compared with control group in the level of total protein, urea, creatinine, (AST and ALT) aparameters but at a significant different (p<0.01) might be show a few increase in (AST and ALT) negligibely compared with control group .

Seroepidemiological aspects of toxoplasmosis among pre-school children in Najaf Province

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 206-211

This study was used to detect anti Toxoplasma gondii IgG and IgM in serum, the records of 154 patients children at the age of less than six years (pre-school age) attending the hospitals and the private laboratories in Najaf/ Iraq during the period from January 2009 to December 2009 were reviewed. Investigation by using VIDAS technique presence of T. gondii antibodies showed that 48% of the patients were infected with the parasite, (27% of male as well as 21% of females) were among the positive cases. The percentage of IgG containing serum (66%) was more those contained IgM (34%). The hematological aspects have some relationship with toxoplasmosis, erythrocytes sedimentation rate, showed a marked increasing level, low concentration of hemoglobin and high white blood cells count. The test for toxoplasmosis during childhood and pre-marriage age especially for girls should be a compulsory test to eliminate or limit the adverse effect of this infection during adulthood.

Iron Status in Patients with Primary Hypertension

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 212-218

Hypertension is a major health problem in adults, and contributes to cardiovascular disease. Iron represents a paradox for human health by being essential for many important biological process, but also having an ability to be harmful in many different. In the present study, an attempt is carried out to define the iron status in Iraqi hypertensive patients. The aim is to obtain a recommendation about the risk of increased iron indices as a marker for subsequent ischaemic heart diseases.
Eighty-eight hypertensive uncomplicated, hypertensive patient, but otherwise have no other systemic diseases patients aged 49±13 years old, were entered into the study. Serum levels of iron,, total Iron Binding Capacity (TIBC), and ferritin were measured while Unsaturated iron-binding capacity (UIBC), estimated total iron body stores (ETIBS), transferrrin saturation percentage (TS%) and transferrin concentration were calculated mathematically.
The results showed a significant difference (p<0.05) in all iron indices of hypertensive patients in comparing with healthy control group except TIBC, UIBC, and transferrin concentrations, which decrease in these patients in comparing with control group. There is a significant increase in serum ferritin and EIBS in male group as compared with female group. While all other iron indices were differ insignificantly between groups.It can be concluded that hypertensive patients have significantly higher level of iron parameters than control group. There is no significant difference between male and female groups in most iron indices except in ferritin and subsequently EIBS levels. Further studies required for other biochemical parameters in larger patients sample size.
Keywords: Iron, TIBC, Ferritin, and Hypertension.

Isolation and identification of bacteria from patients with cholecystits and cholelithiasis undergoing cholecystectomy

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 219-223

Fourty patients were included in this study, which was extending from April 2008 till October 2008. The patients were attending the Al-Sader Teaching Hospital in Al-Najaf province. They were all undergoing clinically and ultrasound examination. 16 of them were with cholecystits and 24 were with cholelithiasis. 11 were males and 29 were females. The age range was 15-65 years. All patients were undergoing cholecystectomy. Bile fluid and pieces of gallbladder were collected from each patient and cultured on routine bacteriological media. Identification of bacterial growth was based on standard bacteriological criteria. The results revealed thatall patients were culture positive. Escherichia coil represent the most common isolates (75%) in cholecystits, and (79.17%) in cholelithiasis, while β-hemolytic streptococci were found in (12.5%) of cholecystits cases and (8.33%) of cholelithiasis cases. Klebsiella spp. were found in (6.25%) of cholecystits and (12.5%) of cholelithiasis cases. Proteus spp. were isolated from (6.25%) of patients with cholecystits. It can be concluded that the isolation rate of Gram's negative bacteria were higher than that of Gram's positive bacteria from patients with cholecystitis and cholelithiasis undergoing cholecystectomy.

Histological study of the effect of Thyroxin and Aqueous extract of Karkade on liver in Swiss male mice

Magazine of Al-Kufa University for Biology, 2011, Volume 3, Issue 1, Pages 224-232

The plant Karkade (Hibiscus sabdariffa L.) has been, for many years used in different countries around the world as a culinary and therapeutic resource, it has been used as antihypertensive, anti-hypercholesterolemia and as diuretic. This study aimed to determine the effect of thyroxin and the aqueous extract of Karkade alone and together on the histological and cytological structure of the liver and the inhibitory effect of the aqueous extract of Karkade on hyperthyroidism.
Forty Swiss albino mice were used in this study; animals were divided into four equal experimental groups. The experimental groups included: control group, animals treated with thyroxin, animals treated with aqueous extract of Karkade and animals treated with thyroxin and with the aqueous extract of Karkade together.
Results showed that the injection of thyroxin has negative effects on the liver, and that the active compounds of aqueous extract of Karkade have an important role in the inhibition of the negative effects of thyroxin
Key words: thyroxin, Hibiscus sabdariffa, karkade, liver, inhibition.