Effect of aqueous extracts of Eucalyptus, Myrtus and Nerium leaves on vegetative parameter and yield and quality of wheat Triticum aestivum L.

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 1-14

An experiment was conducted at Al-Mishkhab Experimental Research Station, to study the effects of water extracts (cold and boiled) of Eucalyptus, Myrtus and Nerium plant leaves on flowering parameters and yield of wheat. Extraction was done by cold water (at room temperature) and boiled water to prepare concentrations of 25% , 50%, 75% and 100% as well as the control treatment (distilled water) on some vegetative growth parameters, productivity and protein content of wheat .
Results showed that boiled water extracts of Eucalyptus, Myrtus and Nerium plant leaves were superior on plant height and its leaves number, productivity and its protein content. Myrtus boiled water leaf extract gave the highest values for the above parameters compared to the other leaf extracts.
It was noticed that growth parameters were inhibited with the increase in the concentration of all leaf extracts except for 25% of Myrtus leaf extract by boiled water which increase the vegetative parameters which in turn reflected in an increase in the productivity by 14.6% compared to control, with protein percentage of 12.06% .

Prevalance of parasitic faunae infection in aquarium fish with investigation of treatment methods

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 21-24

The parasitic founae of gold fish Carassius auratus and blake moly fish
Poecilia sphenops in biological department of Collage of Education for girls in Kufa university . has been studied .Atotal of 200 fishes belong to two species ( 100) gold fishes and (100) moly fishes were collected during the period (August-2008 to April -2009 to study the prevalence and Intensity of infections.
The results of microscopic examination showed that thes fishes were infected with Lernae cyprinaceae . one protozoon parasite Ichthyophthirius multifiliis . one parasite Dactylogyrus agillis . The result sensitivity of these parasite against clove revealed that 5% concentration has astrong inhibiton effect during 5 minuts only .

Effect of Trichoderma harizanum Rafai on the plantation and growth of some rice varieties

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 25-32

This experiment was carried out to study the effect of T. harzianum on the plantation and growth parameters of some varieties of Rice, the study showed high significant differences on the germination percentage in each of Anber33 b, a and yasmin is 90.67% compared with control treatment is 60.67 , 65.67 ,52.67% respectively , also showed the effect in seedling damping-off for same species above is 1.67%, 1%, 2% respectively compared with control treatment is 5%, 6%, 6% respectively and the effect in length of shoot system is 31.67, 32.67 cm respectively for Anber33 b and Mushhab1 compared with control treatment is 21.33 , 20 cm respectively, while length of root system for Anber33 b,a is18.67 , 19.67 cm compared with control treatment is11.33 , 13cm respectively, the study including isolated 20 species of fungi from seeding of Rice, also the Anber 33b recorded the highest rates of present fungi is 300%, whereas Mushhab2 the lowest rate is71.66%, also the results showed positive relation between humidity content and percentage of present fungi.

Effect of pruning and spraying with GA3 , BA on diseases injury and fruit quality characteristics of Fig trees cv. Aswad Diala( Ficus carica L.) during storage

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 33-37

An experiment was conducted in a private orchard at Abbasyia , Najaf Governorate during the growing season of 2009 on fig trees cv. Aswad Diala . The branches at age of one year old were pruned at level of ( 25 , 50 % ) and spraying with concentration of 100 mg/ L of GA3 and BA at 15 March and on 15 / 7 / 2009 , 2 kg of fruits were taken from trees . Fruits of the above treatments were stored at 5C and 80-85% Relative Humidity for 8 days. Results showed that pruning treatments and spraying with GA3 and BA treatments and their interactions caused a significant decrease percentage in the disease of Aspergillus niger , Alternaria tenuis, Penicillium expausum Souring , Alternaria fici, Fusarium solani , total decay , percentage of weight loss and an increase in total soluble solids ,vitamin C and degree of taste in the end of the storage compared to control treatment . There were a significant effect between treatment . Treatment of pruning of branches at age of one year old with 50% + spraying GA3 and BA at concentration 100 mg / L gave a significant effect and the best results for the year of experiment .

The Role of Aspergillus niger in the prgteet of fungi Isolated from Tomato plant and Rhizoctonia solani cause seed decay and damping off Tomato seedlings (Lycopersici esculamtum (Mill)

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 53-61

The experiment was condueted in plant disease lab-at college of Agriculture / university of kufa on march , 22th . 2009 in order to study the effect of Aspergillus miger on contol of fungi which are isolated from tomato plant and Rhizoctonia solani the results showed that it was isolated 8 isolates of fungi accompanied with tomato plants . The highest frequency of fungi was penicillium which is 20.75% , where 95 the least frequency was Rhizopus which was 5.55 % . This expertment indicated that fusarium oxysporum and Rhizoctonia solani were the most virulent to 54.66 and 52% respectively as compared with the control which wsa 82.66 % , and also effected on the others growth Rarameters for example seedlings damp-off preemergence and post emergence the filtering extract of A.niger in habited the gronth og both fungi in the cultural medium which were 5.8 and 7.2 cm respectively as companed with the control which were 7.8 and 8.7 cm respectively the filtering extract of A.niger increased the germination percentage of tomato seeds which were 82.18 nd 79.31% respectively as compared with the contral of 64.33 and 62.17% respectivelyg and also decreased the seesa decay and seedling damp-off as compared with the control.

Effect of N fertilizer and plant density on yield of wheat (Trichicum aestivum)

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 61-63

Field trail conducted at Al-Kalis agriculture project during winter season 2005-2006, 2006-2007. The aim was to study the effect of plant density and rate of N fertilizer on yield of wheat. Wheat variety was (Maxipac), and factorial experiment in R.C.B.D. design used for this experiment. The first factor was sowing rate (15, 20, 25, 30) Kg/d, and the second factor was rate of N fertilizer (0, 15, 30, 45) Kg/d. The experimental unit was (3×4) m2, and the seeds planted in rows 30 cm apart.
Data showed that seed rate (15) Kg/d gave highest yield reached (987, 806) Kg/d for both seasons respectively, which superior on other treatments, and level of N fertilizer (45) Kg/d was superior in yield, which reached (1161, 1047) Kg/d for both seasons, respectively.

Treatment by water hardness on lead toxicity to survival ratios on Barbus sharpeyi (Gunther,1874) juveniles

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 64-67

The effects of water hardness on lead metal toxicity on survival ratios of fish Barbus sharpeyi juvenile were studied. Increasing of water hardness was caused decreased of metal toxicity. Survival ratio has been increased compared with concentrations of lead alone. Survival ratio was increased in low water hardness (Ca 100 ppm), the toxicity of lead was increased in both soft and very hard waters.

Responces of wheat plant variety to Allelopathic phytoexcudates of rice plant

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 72-83

Several experiment were conducted in a college of education laboratories Univercity of Kufa to test responses of wheat plant (Triticum aestivum L.) to Allelopathic phytoexcudates of rice plant (Oryza sativa L.) . Result showed that :
1- Aqueous extract of rice (IPA 1) causation significantly inhibition effected that the aqueous extract of rice (Anber 33) .
2- The rice plant soils significantly inhibition effected in seeds germination and seedling growth of wheat and rice .
3- There are differences between wheat plant and rice plant tolerance ability to allelopathic effected of rice (Anber 33 and Apa 1) in all stage of growth . The autotoxicity between (anber 33) and (Apa 1) appeared very clear-cut but that (Apa 1) significantly effected in Autotoxicity .
4- The rice residues added in (1%) ratio causation significantly reduced in most of treatment ، specially in mature and flowering .
5- The rice plant (Anber 33) more resistance from variety (IPA1) .and the wheat ( maxipak) more resistance from variety ( saberpic )

A Study of Toxic Effects of Aspergillus flavus on Total Leucocytes Count Measurement (W.B.Cs) and Histological Parameters of Female Albino Rates and Capacity of Biological Control on this Effect .

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 84-96

Aims Of study : This study was executed in laboratories of biology department in the college of science it clarifies the toxic effects of the two fungi isolated from the Aspergillus flavus it one ability to produce the aflatoxin but others do not produced it, on some physiological, and histological parameters of white rat female. In addition to evaluation of the efficiency the bioformulation from the Bacillus subtilis bacteria in protecting the animals food from the infection by the fungi A. flavus.
The laboratory experiments proved that the The laboratory experiments proved the bioformulation from the B. subtilis had a high effect in inhibition the radial growth in concentration 1 g / L where the inhibition rate attained 100 % to the both fungi isoluted from the A. flavus . The result of this study demonstrated the presence of different toxic effect from the both fungi isolated from the A. flavus by increasing the level of white blood cells to about 10400 cell / µm3 in rats that injected with filtration of the isolated which produced of the aflatoxin , and 6300 cell / µm3 in rats that injected with filtration from other isolated of the A. flavus compared with of control 3400 cell / µm3 . On the other hand the histological study showed that fungi isolated which produced to the aflatoxin but not treated with bioformulation of the B. subtilis it caused pathological effects on some tissue of the laboratory rats these effects included vascular conducation in the liver tissue , and clear necrosis in some of the liver cells also it causes villous atrophy and characterized by the presence of inflammatory cells and destruction of the mucosa in the small intestine tissue .But the other isolated of the fungi A. flavus dose not caused any pathological effect . also the bioformulation of the B. subtilis it very efficient in the protection the liver and small intestine tissue from the effects of the aflatoxin .This results are considered the primary in Iraq according to the presence scientific references .

Effect of Minimum and Conventional Tillage on Growth and Yield of Two Rice variety (Anbar 33 and Forat )

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 97-103

This research was conducted in Najaf government – Abbasia region at summer season of 2009 .The aim of the research was to find out the effect of minimum tillage system with heavy offset disc harrows and conventional tillage system with mold board plow on the growth and yield properties of two variety of rice Oryza sativa L. ( Anbar 33 and Forat) . The structure of the soil was silty clay loam . Split plot design with RCBD was used to carry out this research with three replications . The results showed that : the conversion from conventional to minimum tillage system with the Anbar 33 variety was led to a significant decreasing in root length , plant height , Productive tillers , Grains per Panicle , Sterility and panicle length which resulted in a significant decreasing in the seed yield of this variety . Whereas the conversion from conventional to minimum tillage with the variety Forat did not show any significant differences in the root length , plant height , Productive tillers , Grains per Panicle , panicle length , Sterility , weight of 1000 grains and rice seed yield , it is mean we can use the minimum tillage with heavy offset disc harrows to prepare the soil for sowing Forat variety of rice crop. Generally the minimum tillage system was exceeded the conventional tillage system in most research properties significantly . Also Forat variety was exceeded Anbar 33 variety in most properties significantly .

Effect of treatment with Castor oil, Latex of fig and Diaminozide in the percentage of decay and quality characters of local Citrus sinensis L.

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 104-108

An experiment was conducted in the College of Education for Girls / Kufa University to investigated the effect of treatment the fruits of local orange with two concentrations of all Caster oil (2and 4)% , Latex of fig (4 and 6)% and Diaminozide ( 500 and 750 )mg/L in percentage of fungal and physiological decay, total decay, percentage of moisture of peel, percentage of dissolve and not dissolved solid material in juice , fruit contents of vit. C , percentage of total dissolved solid material in juice and fruit acidity.
The fruits stored after treatment for three months from 15/11/2008 of 5C.
The result indicated that fruits of treatment gave a significant decrease in fungal and physiological decay and , total decay and increase percentage of moisture of peel, percentage of dissolve and not dissolved solid material in juice , contain of fruits from C vitamin , percentage of total dissolved solid material in juice and fruit acidity compared with control treatment .Treatment with 4% gave a significant increase that gave the lowest percentage to all kind of decay and the highest percentage with other studied parameters after 3 months from storage.

Effect of the Lipopolysaccharide Extracted from Escherichia coli on Growth of Leishmania tropica Promastigote In Vitro

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 109-120

In the present study, the inhibitory effect of lipopolysaccharide extracted from Escherichia coli, on growth of Leishmania tropica promastigote in vitro was investigated.The results showed that the lipopolysaccharide investigated had a marked inhibitory effect, at different concentration on growth of the promastigote .The concentration 100 µgml showed 41% inhibition ratio where as it is about 50% at both 25,250 µgml ,representing L.D.50. The concentration 50 µgml showed 67% inhibition ratio . 75 & 500 µgml showed the highest inhibition ratio as it was 78% and 85% , respectively . Lipopolysaccharide had the same inhibitory effect on number of cells , number of generation and the generation time.

Effect of different level of fertilization of urea on growth and production of rice cv. T85 in Najaf

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 132-137

An experiment was conducted in Mishkab region to study the effect different levels of urea fertilization on three cultivars of rice ( T85 , Yasmin and Intkab ) . three treatment were applied T1 no fertilizer ,T2 : 25 kg/don urea and T3 :50 kg/don urea . The effect of urea level on vegetative growth characteristic ( plant Weight , number of leaves ,leaf area , number of tillers and dry weight ) and production characteristic were studied . the results showed that the interaction between T2 (25 kg/don) and the cultivar gave the highest value of leaf area and dry weight while the highest number of flowerets set was when T2 interacted with T85 cultivar which gave production of 2074 kg/don. Compared with other treatments .

The efficiency test of supernatant of Trichoderma harzyanum fungus on control the larva of khabra beetle (Trogoderma granarium).

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 138-143

The aim of the present study was to test the effect of supernatant concentration of trichoderma harzyanum Fungus on best control. The result showed that the concentration 20 ml/liter of dist .water was the most effective on Mortality percent of larval stages (3rd , 4th , and 5th ) of Khabra insect and it was 7.8% while the other concentrations (10 and 1s )ml /liter did not show any effect . The third larval Stage was the more sensitive. since the mortality rate reached 12.8% while the fifth larval stage was less sensitive (1.7 %) . The effect of treatment period on mortality rate reached the maximum after 6 days of treatment (18.9% )while it was 2.2% after the first and the second day . the interaction between the stages and period gave the 11th larval stage and the 6th day gave higher mortality rate (26.7%).

Efficacy of various chlorhexidine disinfecting agents on the reduction of bacteria and dimensional stability of alginate impression material

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 144-152

Contaminated dental impression with saliva and blood provide a risk of cross infection for the dentist and dental laboratory staff. Microorganism from oral cavity in facts can survive on the impression surface and can be transferred to the stone castsThe aim of the study: To determine the effect of immersion of Chlorhexidine disinfection procedure on the contamination and dimensional stability of alginate impression materialMaterials and methods: 40 volunteer students were selected from Dental Department in the Collage of Medical and Dental Technologies in Baghdad with age ranged from 18-22 years (20 females and 20 males). The students have been classified into 4 groups each group of 10 students.After taking impression of upper dental arch by alginate, the impressions of the first group were immersed in tap water for 10 minutes (the control group). The second group impressions were immersed in Corsodyl mouthwash )Glaxosmithkline group of companies United Kingdom( (Chlorhexidine digluconate 0.2%) for 10 minutes. The third group impressions have immersed in )zak DNV Syria( mouthwash (Chlorhexidine digluconate 0.12%) for 10 minutes. The fourth group impressions were immersed in Almansor mouthwash )Almansor company Iraq Baghdad) (Chlorhexidine digluconate 0.2%) for 10 minutes.ll these impressions were swabbed and inoculated into blood agar plates, plates were incubated and examined for the bacterial colonies growthsThe second part of the study was to investigate the effects of disinfection solutions (different types of Chlorhexidine) on the dimensional stability of alginate impression material. forty specimens were immersed in 4 different solutions groups : group 1 immersed for 10 minutes in tap water (control group), group 2 immersed for 10 minutes in Corsodyl mouthwash (Chlorhexidine digluconate 0.2%) , group 3 immersed for 10 minutes in zak mouthwash (Chlorhexidine digluconate 0.12%) , group 4 immersed for 10 minutes in Almansor mouthwash (Chlorhexidine digluconate 0.2%)Results: the contamination frequency were compared for statistical significance, the alginate (irreversible hydrocolloid) showed contamination in tap water and there was a significant reduction of bacterial growth with Chlorhexidine mouth wash. Corsodyl brand type has superior antibacterial effect over the other two types. There was no significant differences in the dimensional changes test between different types of Chlorhexidine and tap water. Disinfectants techniques by immersion alginate dental impression for 10 minutes caused no significant dimensional change.Conclusion: the use of Chlorhexidine disinfection after taking alginate dental impression is a good measure in reduction of contamination and cross infection and have a minute effect on dimensional stability and is recommended as step in protecting dentist and dental laboratories teams.Key words: Chlorhexidine, alginate impression, cross infection, dimensional stability

The detrimental effect of cigarette smoking on semen parameters and sperm plasma membrane integrity in infertile patients undergoing intra-uterine insemination

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 153-159

صممت الدراسة الحالية إلى تقييم التأثير الضار لتدخين السكائر على متغيرات السائل المنوي و فحص كفاءة غشاء البلازما تحت الضغط التنافذي الواطئ للنطف البشرية (HOS-test) ومعدلات الحمل بعد إجراء التمنية داخل الرحم (IUI) لمرضى العقم.
اشترك في هذه الدراسة مائة شخص يعاني من العقم تم تقسيمهم اعتماداً على تدخين السكائر الى مجموعتين المدخنين (العدد: 55) وغير المدخنين (العدد: 45). تم الحصول على عينة السائل المنوي وتقييم متغيرات النطف والتي شملت تركيز النطف والنسب المئوية لحركة النطف والحركة التقدمية للنطف والشكل الظاهري للنطف السوية و كفاءة غشاء البلازما تحت الضغط التنافذي الواطئ للنطف البشرية اعتماداً على متغيرات منظمة الصحة العالمية (WHO) القياسية. تم تحضير نطف مرضى العقم باستخدام تقنية سباحة النطف للأعلى مباشرةً من خلال الحضن لفترة 30 دقيقة في 5% ثنائي أوكسيد الكاربون وبدرجة 37 مه. أثبتت نتائج الدراسة الحالية ان متغيرات النطف والنسبة المئوية لفحص HOS test ونتائج التمنية داخل الرحم لمرضى العقم غير المدخنين أعلى من مرضى العقم المدخنين. هذا فضلاً عن نتائج متغيرات النطف والنسبة المئوية لفحص HOS test للمدخنين أكثر انحرافا عن المدى الطبيعي لمتغيرات منظمة الصحة العالمية (WHO) القياسية من نتائج غير المدخنين. تم تقييم فروقات غير معنوية لفحص HOS-test بين غير المدخنين والمدخنين. لوحظ من نتائج هذه الدراسة ان تدخين السكائر له تأثيرات ضارة عديدة على وظائف النطف وكفاءة غشاء النطف البلازمي، هذا بالاضافة الى القدرة الاخصابية للنطف. نوصي باجراء دراسات لاحقة لتقييم تأثير تدخين السكائر على تضرر الدنا (DNA) ونوعية الأجنة بعد اجراء عمليات الاخصاب الخارجي ونقل الأجنة (IVF-ET).

Evaluation the effect of mixed different types of water with dental stone on the compressive strength.

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 160-165

The strength of gypsum products is generally expressed in terms of compressive strength and normally measured under compression. It increases rapidly as the materials harden after the initial setting time. However, the amount of water present in the set product definitely affects its strength.This study is designed to evaluate and compare the effect of water used (tap water, distilled water, tap water with powder of stone, distilled water with powder of stone, tap water with impurities of stone, distilled water with impurities of stone) on compressive strength of dental stone.Sixteen (60) specimens were prepared from stone, (10) specimens were considered as a control group (tap water) the remaining specimens were divided into five main group (10) specimens of distilled water, (10) specimens of distilled water with powder of stone (10) specimens of distilled water with impurities of stone (10) specimens of tap water with powder of stone, (10) specimens of tap water with impurities of stone.The result showed that the group of distilled water has increase the compressive strength than control group (tap water), and group of tap water with impurities of stone has decreased compressive strength than control group (tap water).Finally, it can be concluded that the type of surface treatment have an important parameter in the improvement of dental stone.

Tissue Microarray Analysis of HER2 copy number changes in Ovarian cancer

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 166-177

This study was accomplished in Medical University – Pathologoanatomic Department of the Clinic of Thoracic Surgery. Sofia,. Bulgarian, were collected the paraffin blocks, by using new technology Tissue microarrays (TMA) and Fluorescence In Situ Hybridization (FISH)) on different type of ovarian tumors . The method of FISH applied on TMA with HER1 and HER2 specific probes proved itself as the most commonly used and valuable analysis for routine HER2 status detection and copy number changes . The results showed in out of all 603 ovarian carcinomas examined, the number of successfully hybridized for HER2 oncogene is 303 (50.2%) 192 of them are malignancies tumors(179 epithelial and 13 non epithelial ) ,18 low malignant ovarian tumors( just epithelial ), 93 – Benign ovarian tumors(77 epithelial and 16 non epithelial ) . We established HER2 amplification in malignancies tumors only 20 (10.41%) ,19 (10.61%) epithelial and in 1 (7.69%) non epithelial ) . There is no amplification in tumors with low malignant potential and Benign ovarian tumors. While we found HER2 gains in malignancies tumors , low malignant ovarian tumors and Benign ovarian tumors only 19 , malignancies 14 about (7.29%) ,low malignant 2 (11.11%) and in Benign ovarian tumors 3 (3.22% ).
Epithelial malignant tumors among the highest incidence of HER2 amplification was detected in Clear cell carcinomas 2 (28.57%) and rank them Endometrioid 2 (16.66%), Serous 9 (10.46%), undifferentiated tumors 2 (11.11%) and mixed(combined) 2 (11.76%), Mucinous 1 (6.25%) and Unclassification 1 (4.55%). HER2 amplification was found in 1 of 13 non epithelial malignant tumors, Sex-cord stromal tumours 1 (7.69%). HER2 gains in malignancies tumors was detected in serous 5 (5.81%) ,endometrioid 1 (8.33%) , undifferentiated 2 (11.11%) , mixed (combined) 2 ( 11.7%) and mucinous1 (6.25%), in low malignant ovarian tumors was detected HER2 gain only in mucinous 2 (11.11%) and Benign ovarian tumors only in serous 3 (7.69% ).Were preserved at the grouping of tumor samples according to the WHO classification


Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 0-0

This study was designed to evaluate the effects of cadmium chloride (CdCl2) on male reproductive system of Swiss albino mice. Forty male Swiss albino mice were divided randomly in two groups ( treatment & control groups ) . All animals were subcutaneous injected every four days for four weeks. The treatment group was injected with 2.5 mg/kg body weight from cadmium chloride solution, while the control group injected by normal saline solution. All animals of the control and treatment groups were weighted and sacrificed after the end of the experimental period, blood samples were collected and the testes, epididymes and seminal vesicles were dissected out and weighted. Hematoxylin-Eosin stained sections were prepared from these organs for light microscopic investigation. Body weight means of CdCl2-treated mice was showed significant reduction compared with control group (p > 0.001). However, combined testicular-epididymal and seminal vesicular weight means of CdCl2-treated mice were significant decreased compared by control group ( p > 0.001 , p > 0.001 respectively) and there were a significant decrease in levels testosterone compared with control ( p > 0.001 ). Stained histological sections of testes and epididymis of CdCl2-treated group reveal a degradation of seminiferous tubules and deformity in spermatogenesis as well as absence of spermatozoa, decrease in the seminal vesicular secretion was noted in CdCl2-treated mice.

Antimicrobial Activity of Bacteriocin Produced by Weissella cibaria NRIC0136

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 188-196

A total of 1070 isolates of lactic acid bacteria were obtained from 50 samples of different sources (fermented foods 30 samples, chicken intestine (10 samples) and human intestine and vagina (10 samples). Three hundred isolates were isolated from Baghdad, Iraq (220 isolates from human intestine and vagina and 80 isolates from fermented foods) and the others from Bangkok (Thailand). These isolates were screened for bacteriocin production using the agar well diffusion method and Shigella dysenteriae DMST 15110 was used as an indicator strain. Seventy two isolates gave clear inhibition zones against growth of indicator strain.
One isolate , F14 (from Thailand fermented fish) that produced largest inhibition zone against the indicator strain was chosen for further study, and identified as a Weissella cibaria NRIC0136 by morphological, physiological properties and PCR amplification, sequencing and comparison of 16S rRNA gene. The neutralized cell free supernatant of W. cibaria NRIC0136 inhibited growth of a number of standard strains of pathogenic microorganisms including Listeria monocytogenes DMST 17303, Enterococcus faecalis ATCC 29212, Salmonella sp DMST 22842 and Shigella dysenteriae DMST 15110. The optical density at 660nm (2.253) and inhibition zone (18 mm) against the indicator strain, Shigella dysenteriae DMST 15110 were maximum when growth temperature employed was 30°C.

Relationship between Dehydroepiandrosterone Sulfate and Ischemic Heart Disease in Men

Shaimaa Mahdi A. Jawad; Sabah A.R. Al-Obaidi; Yahya K. Al-Sultani

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 197-206

Thirty two patients with ischemic heart disease (aged 40- 69 years) involved in this study during their admission Ibn-Albitar, Ibn-Alnafees Hospitals Baghdad and the Iraqi Center for Heart Surgery, Medical City. Age matched twenty seven healthy men also included as control group. The results obtained are: serum triglycerides (TG) and very low density lipoprotein (VLDL) shows a significant (p<0.05) increase in patients with I.H.D as compared with control group in age range 50-59 years. As well as, no significant correlation was found in I.H.D and control groups between dehydroepiandrosterone sulfate (DHEA-S) and lipid profile, although there was a slight negative correlation between DHEA-S levels and each of TG and VLDL in I.H.D group.

Genital tract infections among pregnant women

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 207-212

One hundred fifty six high vaginal swabs were collected from 126 pregnant women and 30 non-pregnant women . Isolation and identification of microorganisms based on standard bacteriology and biochemical procedure. The results showed that 37.3% , 28.5% , 5.5% , and 6.3% of pregnant women had bacterial , Candida albicane , Trichomonas vaginalis and Garderella vaginalis infections respectively. Which are higher than their counterpart in non-pregnant women. As well as presence of vaginal discharge , case history of abortion and intrauterine contraceptive device insertion were positively correlated with increase infection rates. Routine vaginal swab examination should be introduce as mandatory procedure for all pregnant women, particularly those under certain risk factors at antenatal health care centers.

The Effect of smoking in different durations on some of the fertility parameters for the patients attending an infertility center of Al-Sader teaching hospital in governorate of Al- Najaf AL-Ashraf

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 213-223

This study have been performed in June 2009 in the center of infertility of Al-Najaf AL –Ashraf governorate . The aim of this study is to investigate the relationship between cigarette smoking and semen parameters for 40 specimens included control provided from men who are smoking in different durations were divided into three yearly durations (5-9),(10-14)and (15-20)years regardless of the number of cigarettes per day , each group contain ten samples. Semen analysis was performed manually according to the World Health Organization(WHO) criteria standard guidelines and the results are compared with control. The results were showed that sperm parameters quality in the last group of smokers was lower than others so that it has been represented the most effect of group),therefore it appears that smoking effects on the mainly sperm parameters such as sperm count, sperm morphology and motility additional to liquefaction time and semen volume , this effect is represented by reduced semen quality and quantity in hich sign to arise the risk of idiopathic male infertility.

Record birds Corvus corone as new host for the cestoda Cotugni intermedia and Raillietinia micracantha in Al- Najaf Al-Ashraf;Iraq

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 224-230

During the period of the present study (June - October -2008)
Corvus corone were . found infected with Cestoda Cotugnia intermeda and Raillietina micracantha with incidence of(0.75%,0375%) respectively.In this study Corvus corone regarded as anew host for these two cestodes

The efficacy of 690 nm diode laser and diclofenac sodium in reduction of signs and symptoms after surgical removal of vertically impacted mandibular third molars

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 230-237

The signs and symptoms after surgical removal of the impacted mandibular third molar tooth can include pain, trismus and swelling; there are many modalities to treat these signs and symptoms like pain killer and non steroidal anti-inflammatory drugs. Low power laser therapy can be one of these modalitiesThe aim of the study is to investigate the effects of low power laser therapy, diclofenac sodium or in combination in reduction of post operative signs and symptoms.Material and methods: A total of 40 patients requiring surgical removal of mandibular third molars were divided in 4 groups of 10 patients in each group: the first group treated with low power laser therapy, group 2 was treated with diclofenac sodium, group 3 was treated with combination of low power laser and diclofenac sodium and group 4 was treated with placebo.Results: the postoperative pain records for group 1, group 2 and group 3 were pain records less than that for group 4. The mean of postoperative mouth opening measurements records for group1, group2 and group 3 were more than the group 4. Also the mean of postoperative swelling records for group1, group2 and group 3 were less than the group 4These results may agree with most researchers in proving that Low power laser therapy can be beneficial in the reduction of post-operative signs and symptoms; however the methods of application and the doses of laser power need further investigations.In conclusion low power laser therapy alone or with combination with Diclofenac sodium medication can be good procedure for the reduction of postoperative signs and symptoms after surgical operation of removal of mandibular third molar. Diclofenac sodium medication alone was superior to Low power laser therapy alone .

Comparative study of effect garlic and anion extract on the growth of some gram negative bacteria

Dr.wafa S. Alwazni; Dr.mayada f.Drwesh

Magazine of Al-Kufa University for Biology, 2010, Volume 2, Issue 1, Pages 238-244

During this study, a comparison of antimicrobial effects of garlic and onion extract on some gram negative bacteria were assayed in vitro. Primarily, each of these microorganisms including Escherichia coli, Salmonella typhi, Klebsiella pneumonia and Pseudomonas aeruginosa were isolated and diagnosed from patients suffering from different infection cases. This step was followed by preparation of aqueous extracts from onion and garlic. Furthermore, the activity of the two extracts on those microorganisms was tested by using different garlic extract concentrations, and application cup–plate diffusion and total count methods. It was noticed that increasing garlic aqueous extract concentration, would lead to a notable increase in inhibition zone diameter, compared with control. However the inhibition zone diameter was about 28 mm for Salmonella typhi, at concentration of 500mg/0.5ml of garlic extract was used; while the diameter was about 26–30–16 mm for the same concentration for each of K. pneumonia, Escherichia coli and P. aeruginosa respectively. Moreover a comparison was done between these results and that obtained when using onion aqueous extract. It was observed that the activity of garlic was more than onion which gave inhibitory zone diameter of about 25–16–17 mm to each of S. typhi, K. pneumonia and E. coli respectively , at concentration of 500mg/0.5ml; but the P. aeruginosa was unaffected. Other observation was show that there is adecrease in number of isolated microbial cells by increasing both extract concentrations, hence when treating the bacteria with garlic extract of 500mg/0.5ml concentration, the number of cells forming colonies were 62 10 , 71 10 , 12 10 and 13 10 cell/ml for each of S. typhi, K. pneumonia, E. coli and P. aeruginosa respectively. In the other hand, the number of colony forming unit for the same bacteria were 16 10 , 31 10 , 1 10 and 8 10 cell/ml when using onion extract of 500mg/0.5ml concentration.