Evaluation the interplay of T-helper 22 and T-helper17 in patients with breast cancer

Dina M.R. Alkhaf; Raghda S. M. AlOmari; Ziad M. Alkhozai

Magazine of Al-Kufa University for Biology, 2016, Volume 8, Issue 3, Pages 273-283

The interplay between Th-17 and Th-22 cells plays vital role in tumor immunity . Therefore, this study aimed to focus on these cells in patients with breast cancer. The results of this study showed that there were a highly significant increment in concentrations of IL-17A(139.5±17pg/ml) compared with control group which was (41.33±11.3pg/ml) and showed results were a highly significant elevation in concentration of IL-23P19 (192.73±22.3pg/ml) while control group was (121.41±14.7pg/ml). Also The results showed a highly significant increment in concentration of IL-22 (137.25±24.6pg/ml) compared to control group which was (77.67±13.7pg/ml) and also in TNF-α concentration was (200±23.7pg/ml) compared with control group which was (10.5±7.3pg/ml). Molecular findings recorded a significant elevation in the levels of AP-1 gene expression were Fos 18.76±7.8 in patients group and 9.05±1.12 control group, also the values of JunD gene 15.77±9.43 and 6.18±0.112, and lastly JunB 23.223±11.34 in comparison to control group7.33±3.76.Overall findings revealed considerable inflammatory response by Th-17 and Th-22 and remarkable AP-1 gene expression.

Application of Tissue Microarray Technology to detect expression of Ki-67 in breast carcinoma patients

Saeed hilal khudhair; Radhia hussain fadel; Wafaa Fadhil Hamad

Magazine of Al-Kufa University for Biology, 2013, Volume 5, Issue 1, Pages 171-179

Breast cancer is the most common cancer of women worldwide. It is a heterogeneous disease. The incidence rate of this disease increased in the last years in Iraq. The current study was conducted to study the immunohistochemical for the breast tumors by using tissue microarray (TMA) technique. The mean age of the Iraqi breast cancer patients in our study was (50.32±11.85) years old; ranging from 23-80 years old.Formalin-fixed, paraffin-embedded (FFPE) samples were used in this study. Two TMAs recipient blocks were designed and constructed for samples.Both two TMAs contain 30 samples of Iraqi breast cancer patients and normal kidney tissue for control. Each donor sample were represented in duplicate, cores were transferred from each donor patient sample to the recipient block, sections from the sample slides cutting and potted on charge slide then stained these sections with hematoxylin and Eosin (H and E) stained then immunohistochemically (IHC) studied for Ki-67 for the TMAs.
The results showed that 35 out of 60 cases was positive expression for Ki-67, and the most cases were grade II for expression all of Ki-67. While results of positive lymph node status were found in 19 cases out of 35 was positive expression for Ki-67. The study revealed that there was a significant relationship between the Ki-67 and histological grade and lymph node status. The results indicated that the Iraqi breast cancer cases used in this study were phonotypical more aggressive. We utilized TMA sections for the validation of the Ki-67. In conclusion, TMA was successfully used as a rapid, economic and effective method for IHC studies.